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Apoptosis & Viability Assays

Biotium offers a wide selection of assay kits for cell viability and cell death for microplate reader, flow cytometry or fluorescence microscopy. Products include:

The NucView® Caspase-3 Substrate comes in 3 colors, and measures caspase activity in real time, in live cells. MitoView™ 633 can measure the decrease in mitochondria membrane potential that occurs in early apoptosis. CF® Dye Annexin V conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes. Live-or-Dye dead cell stains for flow or microscopy. Fluorescence- and absorbance-based microplate viability assays such as MTT, XTT, and resazurin. ATP-Glo Bioluminometric Cell Viability luciferase assay. ViaFluor® Cell Proliferation Kits.

Learn more about our wide selection of assays for cell proliferation, cell viability, and apoptosis.

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    Live-or-Dye NucFix™ Red Staining Kit

    From: $60 Sizes: 50 assays, 200 assaysCatalog #: 32010, - 32010-TView allHide

    Live-or-Dye NucFix™ Red is a unique, cell membrane impermeable dye that specifically stains the nuclei of dead cells.

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    Live-or-Dye NucFix™ Red is a unique, cell membrane impermeable dye that specifically stains the nuclei of dead cells.

     

    Live-or-Dye™ Fixable Viability Staining Kits

    From: $63 Sizes: 50 assays, 200 assaysCatalog #: 32002-T, 32003-T, 32009-T, 32004-T, 32005-T, 32006-T, 32007-T, 32008-T, 32002, 32003, 32009, 32004, 32005, 32006, 32007, - 32008View allHide

    Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.

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    Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.

     

    MitoView™ Dyes

    From: $40 Sizes: Trial size (50 ug), 20 x 50 ugCatalog #: 70070, 70054, 70055, 70070-T, 70054-T, 70055-T, 70068, - 70068-TView allHide

    MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

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    MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

     

    ViaFluor® SE Cell Proliferation Kits

    From: $50 Sizes: Trial size (1 vial), Set of 10 vialsCatalog #: 30050, 30068, 30086, 30068-T, - 30086-TView allHide

    ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and are used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. The labeling withstands fixation and permeabilization for immunostaining.

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    ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label the cell cytoplasm, and are used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. The labeling withstands fixation and permeabilization for immunostaining.

     

    Annexin V CF® Dye Conjugates, Azide-Free, Lyophilized

    From: $60 Sizes: 5 ugCatalog #: 29012R-5ug, 29009R-5ug, 29083R-5ug, 29005R-5ug, 29004R-5ug, 29010R-5ug, 29011R-5ug, 29008R-5ug, 29014R-5ug, 29003R-5ug, - 29069R-5ugView allHide

    Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments.

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    Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments.

     

    Annexin V Conjugates

    From: $89 Sizes: 100 uL, 500 uL, 0.5 mLCatalog #: 29001, 29002, 29003, 29004, 29005, 29008, 29009, 29010, 29011, 29012, 29013, 29014, 29069, 29045-100uL, 29045-500uL, 29057-100uL, 29057-500uL, - 29083View allHide

    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

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    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

     

    Annexin V Near IR CF® Dye Conjugates

    From: $263 Sizes: 25 ugCatalog #: 29070, 29006, 29007, 29046, 29047, 29078, - 29082View allHide

    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available labeled with our superior near-IR fluorescent dyes.

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    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available labeled with our superior near-IR fluorescent dyes.

     

    Apoptosis and Necrosis Quantitation Kit Plus

    From: $173 Sizes: 50 assaysCatalog #: 30065

    The Apoptosis & Necrosis Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy

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    The Apoptosis & Necrosis Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy

     

    Apoptotic, Necrotic and Healthy Cells Quantitation Kit Plus

    From: $173 Sizes: 50 assaysCatalog #: 30066

    The Apoptosis, Necrosis & Healthy Cells Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

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    The Apoptosis, Necrosis & Healthy Cells Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

     

    CF®488A Annexin V and 7-AAD Apoptosis Kit

    From: $210 Sizes: 100 assaysCatalog #: 30060

    This kit contains CF488A-Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells.

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    This kit contains CF488A-Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells.

     

    CF®488A Annexin V and PI Apoptosis Kit

    From: $210 Sizes: 100 assaysCatalog #: 30061

    This kit contains CF488A-Annexin V to stain apoptotic cells and PI to stain necrotic cells.

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    This kit contains CF488A-Annexin V to stain apoptotic cells and PI to stain necrotic cells.

     

    Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V

    From: $341 Sizes: 50 assaysCatalog #: 30030, 30067, - 30073View allHide

    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugated with your choice of red or far-red dye. The kit is used for studying two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.

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    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugated with your choice of red or far-red dye. The kit is used for studying two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.

     

    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO

    From: $63 Sizes: Trial size (10 uL), 100 uLCatalog #: 10405, - 10405-TView allHide

    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 405 Caspase-3 Substrate, 1 mM in DMSO, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    NucView® 405 Caspase-3 Substrate, 1 mM in PBS

    From: $63 Sizes: Trial size (10 uL), 100 uLCatalog #: 10407-T, - 10407View allHide

    NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

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    NucView® 405 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry.

     

    NucView® 488 and MitoView™ 633 Apoptosis Assay Kit

    From: $341 Sizes: 100 assaysCatalog #: 30062

    This kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

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    This kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

     

    NucView® 488 and RedDot™ 2 Apoptosis and Necrosis Kit

    From: $299 Sizes: 100 assaysCatalog #: 30072

    NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.

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    NucView®488 and RedDot™2 Apoptosis/Necrosis Assay Kit contains NucView®488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity.