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Apoptosis & Viability Assays

Biotium offers a wide selection of assay kits for cell viability and cell death for microplate reader, flow cytometry or fluorescence microscopy. Products include:

The NucView® Caspase-3 Substrate comes in 3 colors, and measures caspase activity in real time, in live cells. MitoView™ 633 can measure the decrease in mitochondria membrane potential that occurs in early apoptosis. CF® Dye Annexin V conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes. Live-or-Dye dead cell stains for flow or microscopy. Fluorescence- and absorbance-based microplate viability assays such as MTT, XTT, and resazurin. ATP-Glo Bioluminometric Cell Viability luciferase assay. ViaFluor® Cell Proliferation Kits.

Learn more about our wide selection of assays for cell proliferation, cell viability, and apoptosis.

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    Live-or-Dye NucFix™ Red Staining Kit

    From: $62 Sizes: 50 assays, 200 assaysCatalog #: 32010, - 32010-TView allHide

    A red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected in flow cytometry and microscopy.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    A red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected in flow cytometry and microscopy.

     

    Live-or-Dye™ Fixable Viability Staining Kits

    From: $65 Sizes: 50 assays, 200 assays, 250 assaysCatalog #: 32016, 32017, 32002-T, 32014-T, 32003-T, 32009-T, 32004-T, 32012-T, 32015-T, 32005-T, 32007-T, 32006-T, 32013-T, 32008-T, 32011-T, 32002, 32014, 32003, 32009, 32004, 32012, 32005, 32006, 32015, 32007, 32013, 32008, - 32011View allHide

    Kits to covalently label dead cells, allowing cells with permeable plasma membranes to be excluded from analysis in flow cytometry. A wide variety of dye options for standard flow, spectral flow, and fluorescence microscopy.

    Product
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Kits to covalently label dead cells, allowing cells with permeable plasma membranes to be excluded from analysis in flow cytometry. A wide variety of dye options for standard flow, spectral flow, and fluorescence microscopy.

     

    NucSpot® 470 Nuclear Stain, 1000X in DMSO

    Sizes: 20 uL, 200 uLCatalog #: 40083-T, - 40083View allHide

    A cell membrane-impermeant, nuclear-specific green fluorescent DNA stain.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    A cell membrane-impermeant, nuclear-specific green fluorescent DNA stain.

     

    RedDot™2 Far-Red Nuclear Stain, 200X in DMSO

    From: $77 Sizes: 25 uL, 250 uL, 1 mLCatalog #: 40061, 40061-1, - 40061-TView allHide

    A far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for fixed cell nuclear counterstaining with minimal cytoplasmic RNA staining.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    A far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for fixed cell nuclear counterstaining with minimal cytoplasmic RNA staining.

     

    ViaFluor® SE Cell Proliferation Kits

    From: $50 Sizes: Trial size (1 vial), Set of 10 vialsCatalog #: 30050, 30068, 30086, 30068-T, - 30086-TView allHide

    Fixable cytoplasmic stains for monitoring cell division by flow cytometry. The dyes can also be used to track cell populations in co-culture.

    Dye Color
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Fixable cytoplasmic stains for monitoring cell division by flow cytometry. The dyes can also be used to track cell populations in co-culture.

     

    Annexin V CF® Dye Conjugates, Azide-Free, Lyophilized

    From: $62 Sizes: 5 ugCatalog #: 29085R-5ug, 29012R-5ug, 29009R-5ug, 29083R-5ug, 29005R-5ug, 29004R-5ug, 29010R-5ug, 29011R-5ug, 29008R-5ug, 29014R-5ug, 29003R-5ug, - 29069R-5ugView allHide

    Preservative-free Annexin V conjugates are compatible with real-time staining of apoptotic cells for live cell imaging, fluorescence microscopy, or flow cytometry.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Preservative-free Annexin V conjugates are compatible with real-time staining of apoptotic cells for live cell imaging, fluorescence microscopy, or flow cytometry.

     

    Annexin V Conjugates

    From: $95 Sizes: 100 uL, 500 uL, 0.5 mLCatalog #: 29085, 29001, 29003, 29004, 29005, 29008, 29009, 29010, 29011, 29012, 29013, 29014, 29069, 29045-100uL, 29045-500uL, 29057-100uL, 29057-500uL, - 29083View allHide

    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

     

    Annexin V Near IR CF® Dye Conjugates

    From: $279 Sizes: 25 ugCatalog #: 29070, 29006, 29007, 29046, 29047, 29078, - 29082View allHide

    Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.

    Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    25 ug
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    Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.

     

    Apoptosis and Necrosis Quantitation Kit Plus

    From: $178 Sizes: 50 assaysCatalog #: 30065

    A convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    A convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

     

    Apoptotic, Necrotic and Healthy Cells Quantitation Kit Plus

    From: $183 Sizes: 50 assaysCatalog #: 30066

    A convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    50 assays
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    A convenient assay for quantifying apoptotic (green), necrotic (red), and total (blue) cells within the same cell population by flow cytometry or fluorescence microscopy.

     

    CF®488A Annexin V and 7-AAD Apoptosis Kit

    From: $222 Sizes: 100 assaysCatalog #: 30060

    This kit contains CF®488A Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells for flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 assays
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    This kit contains CF®488A Annexin V to stain apoptotic cells, and 7-AAD to stain necrotic cells for flow cytometry.

     

    CF®488A Annexin V and PI Apoptosis Kit

    From: $222 Sizes: 100 assaysCatalog #: 30061

    This kit contains CF®488A Annexin V to stain apoptotic cells green and propidium iodide (PI) to stain necrotic cells red for fluorescence microscopy or flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 assays
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    This kit contains CF®488A Annexin V to stain apoptotic cells green and propidium iodide (PI) to stain necrotic cells red for fluorescence microscopy or flow cytometry.

     

    Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate & Annexin V

    From: $362 Sizes: 50 assaysCatalog #: 30030, 30067, - 30073View allHide

    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.

    Conjugation
    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    50 assays
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    These kits provides two apoptosis markers, our novel NucView® 488 Caspase-3 Substrate and Annexin V conjugate for detecting caspase-3 activation and phosphatidylserine (PS) translocation.

     

    NucView® 488 & MitoView™ 633 Apoptosis Assay Kit

    From: $351 Sizes: 100 assaysCatalog #: 30062

    No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 assays
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    No wash, homogeneous assay for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using fluorescence microscopy, real-time imaging, or flow cytometry.

     

    NucView® 488 & RedDot™ 2 Apoptosis & Necrosis Kit

    From: $308 Sizes: 100 assaysCatalog #: 30072

    Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    100 assays
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    Homogeneous, no wash assay for detection of caspase-3/7 activity with green fluorescence and dead cell staining with far-red fluorescence, for fluorescence microscopy or flow cytometry.

     

    NucView® 488 Caspase-3 Assay Kit for Live Cells

    From: $98 Sizes: Trial size (25 assays), 100 assaysCatalog #: 30029, - 30029-TView allHide

    Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.

    Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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    Green fluorescent caspase-3/7 substrate for detecting apoptosis in intact cells by confocal microscopy, flow cytometry, or real-time live cell imaging.