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Are TrueBlack® Lipofuscin Quenchers cell permeable, and can they be used on live cells? Have they been used for flow cytometry?

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TrueBlack® Plus Lipofuscin Autofluorescence Quencher is cell membrane permeant, and at low concentrations it is not toxic to live cells. In live cells, the dye accumulates in lysosomes and other intracellular vesicles. Therefore, it may or may not be useful for quenching live cell autofluorescence for flow cytometry or microscopy, depending on the source and localization of the autofluorescence and target probes in the specific cell type.

The concentrations of TrueBlack® Plus that we’ve tested in live cells are much lower than those used for quenching in tissue sections, 0.001X to 0.005X (corresponding to 1:40,000 to 1:8,000 dilution of the 40X stock). Incubation can be done in cell culture medium for 30 minutes to several days, though toxicity may vary between cell types.

Our original TrueBlack® Lipofuscin Autofluorescence Quencher (catalog no. 23007)  is not soluble in aqueous buffers or media, and therefore is not compatible with live cell staining.

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