EverBrite™ Mounting Medium & CoverGrip™ Coverslip Sealant
Yes, EverBrite™ mounting media are compatible with a wide range of dyes. Unlike VectaShield®, EverBrite™ won’t interfere with cyanine-based fluorophores like Alexa Fluor® 647, Cy®3, or Cy®5.
AlexaFluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare; VectaShield is a registered trademark of Vector Laboratories.
EverBrite™ Mounting Medium (23001) and EverBrite™ Mounting Medium with DAPI (23002) both have a refractive index of 1.46. EverBrite™ Hardset Mounting Medium (23003) and EverBrite™ Hardset Mounting Medium with DAPI (23004) both have an initial refractive index of 1.38, which increases to 1.42 after 24 hours of curing, and 1.46 after 4 days, after which it remains constant
EverBrite™ wet-set mounting medium is an aqueous, glycerol-containing medium. It can be removed from samples by washing several times with PBS or other buffer. However, washing with PBS will not remove DAPI staining.
For coverslips mounted on slides with EverBrite™ Hardset or EverBrite™ wet-set medium, the slides can be soaked in PBS in a slide jar for several hours with gentle agitation until the coverslip slides off (avoid prying or pulling the coverslip, which could damage the sample). Then wash the slide several times to remove any remaining mounting medium. If the coverslips have been sealed with CoverGrip™ Coverslip Sealant, soaking in PBS will remove the sealant as well. Other methods may be required to remove other types of coverslip sealants.
CoverGrip™ is designed to replace nail polish for permanent sealing of wet-mounted coverslips. It can be removed by soaking slides in buffer (see the product information sheet for details). CoverGrip™ is not designed to create a removable temporary seal for hybridization.
EverBrite™ mounting medium does show high background fluorescence with violet excitation. The background is more prominent when imaged using epifluorescence using UV excitation and a DAPI filter cube, but is generally quite low in confocal imaging using 405 nm laser.