TrueBlack® Background Reducers
TrueBlack® lipofuscin quencher should be mounted using an aqueous-based mounting medium. It cannot be used with organic solvent-based mounting media like Permount™ or DPX.
The TrueBlack® lipofuscin quencher is compatible with the following mounting media (with or without DAPI):
EverBrite™ Mounting Medium
EverBrite™ Hardset Mounting Medium
Slowfade® Gold (Thermo Fisher Scientific)
Prolong® Gold (Thermo Fisher Scientific)
VECTASHIELD® (Vector Laboratories)
VECTASHIELD® Hardset (Vector Laboratories)
Fluoromount-G® (Southern Biotech)
TrueBlack® Lipofuscin Quencher can be used with the TrueBlack® IF Background Suppressor (Permeabilizing) kit in an immunofluorescence staining workflow, to reduce background from both non-specific antibody and dye binding, and autofluorescence arising from the sample itself. Because TrueBlack® IF reagents contain detergent, the TrueBlack® lipofuscin quencher would need to be used after immunostaining (Post-treatment protocol) as the latter is not compatible with detergents present in the IF reagents.
If immunostaining is performed with buffers lacking detergents, the TrueBlack lipofuscin quencher may be used before (Pre-treatment protocol) or after immunostaining.