Protein Gel Stains
Use of pre-stained protein markers can quench lumitein fluorescence. Switching to an unstained marker should resolve the issue.
Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step as described in the protocol (PI-21002).