Yes, exosomes and EVs can be stained simultaneously with an ExoBrite™ EV Membrane Stain and a fluorescent antibody.
With purified exosomes, we have seen good results when exosomes were stained in 1 mL of 1X ExoBrite™ plus 1 ug fluorescent antibody.
With bead-bound exosomes we have found that sequential staining with first antibody and then ExoBrite™ EV Membrane Stain, with a wash step in between, gives the best results. When the staining is done simultaneously, the antibody signal is unaffected but the ExoBrite™ signal is reduced by ~ 25 %. This was observed when bead-bound exosomes were stained in 100 uL of 10X ExoBrite™ plus 1 ug fluorescent antibody; using different ratios of ExoBrite™ and antibody may give different results.
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