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Can I stain my cells with CellBrite® Cytoplasmic Membrane Dyes at room temperature (or lower) instead of 37°C?

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Yes, CellBrite® Cytoplasmic Membrane Dye staining can be done at room temperature or lower, instead of 37°C, this may reduce dye internalization during staining. Be aware that staining at 4°C may result in dye precipitation or uneven staining.

CellBrite® and MemBrite® Stains were originally developed for staining mammalian cells in culture, but some of the stains also have been validated for other organisms and applications. If you are staining a non-mammalian cell type or organism that grows at a temperature below 37°C, please see our Tech Tip: Researching Applications for Membrane Dyes. For dyes to stain yeast or bacteria membranes, see Cellular Stains in Different Organisms.


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