No. Fixation, freezing, sectioning, or dissociation of tissues can affect the PS on the outer leaflet and compromise membrane integrity. Both Annexin V and Ethidium Homodimer III rely upon the presence of intact membranes in healthy cells to accurately distinguish healthy cells from apoptotic or necrotic cells. To detect apoptosis in fixed cells and tissues we recommend our TUNEL kits. Currently we are not aware of a fluorescent probe that specifically detects necrotic cells in fixed tissues. Necrotic cells in tissue sections are identified based on morphological criteria.View more FAQs
Can I use the Apoptosis and Necrosis kit for staining fixed cells or tissues?
Apoptosis, Necrosis, and Cell Viability KitsCellBrite® & MemBrite® Membrane & Cell Surface StainsCF® DyesDNA Quantitation KitsDNAzure® Visible DNA Gel StainEvaGreen® Dye and Master MixesEverBrite™ Mounting Medium & CoverGrip™ Coverslip SealantExosome & EV StainingGelRed® and GelGreen® Nucleic Acid Gel StainsGelRed® and GelGreen® TroubleshootingIon Indicators & ChelatorsLuciferase AssaysMicrobiology: Dyes for bacteria and yeastMix-n-Stain™ Antibody Labeling KitsNucView® Caspase-3 Enzyme SubstratesOrganelle & Cytoskeleton StainsOther DNA/RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)