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What should I do if I see clumping or precipitates seen on sample treated with TrueBlack® Lipofuscin Autofluorescence Quencher?

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The TrueBlack® lipofuscin quencher is hydrophobic in nature. Certain experimental conditions can cause the quencher to leave precipitates or clumps on the treated sample which can interfere with imaging. We recommend heating the vial of the stock solution of TrueBlack®, 20X in DMF to 70ºC for 5 min. to avoid this. Diluting and storing TrueBlack as a bulk 1X solution in 70% ethanol can also help.


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