Do I have to use two aliquots of lysate for the dual Firefly & Renilla Luciferase Assay Kit? Promega’s Dual Luciferase® Reporter Assay allows for the consecutive readings from the same sample lysate.
No, our new Firefly & Renilla Single Tube Assay Kit allows you to measure firefly and Renilla luciferase activities sequentially in a single sample of lysate.