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Could I be losing some of my apoptotic cells during processing of cells from an adherent culture?

You may wish to process floating and detached cells separately – collect the washes and spin them down and then stain those cells using the protocol for suspension cells. Alternatively you may collect floating cells, detach the adherent cells with trypsin (without EDTA), pool the cells together, and use the suspension cell protocol for staining.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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