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I am using fewer cells than suggested in the Apoptosis and Necrosis kit protocol, should I adjust the amount of dyes in the assay?

No, the concentration of Annexin V and Ethidium Homodimer III should be kept constant regardless of cell number. However, the staining concentrations can be increased or decreased if necessary to optimize staining.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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