Is illumination even across all positions in the PMA-Lite™ device?

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Each tube position on the PMA-Lite™ is illuminated by the three LED bulbs. We haven’t tested positional variability, but it is likely that the illumination varies slightly between positions and between devices. However, the illumination at each position is exceedingly bright, far in excess to what is required for photocrosslinking of the viability dyes EMA, PMA or PMAxx™ to nucleic acids, so any variability should not significantly affect the vPCR results.

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