Is the TrueBlack® Lipofuscin Autofluorescence Quencher compatible with in situ hybridization workflows?

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TrueBlack® Lipofuscin quencher can be used with in situ hybridization. There are several references in literature describing this (a few are listed below).

https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-14484-9.

https://doi.org/10.1101/2020.02.03.931618.

https://doi.org/10.1186/s13195-019-0469-0.

If solvents or buffers containing detergents are used, TrueBlack treatment should be performed after these steps, preferably after rinsing the section with PBS. TrueBlack® may also precipitate out due to the high salt SSC wash buffers used. Rinsing the sections in PBS prior to TrueBlack treatment may help minimize this.

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