TrueBlack® Lipofuscin quencher can be used with in situ hybridization. There are several references in literature describing this (a few are listed below).
If solvents or buffers containing detergents are used, TrueBlack treatment should be performed after these steps, preferably after rinsing the section with PBS. TrueBlack® may also precipitate out due to the high salt SSC wash buffers used. Rinsing the sections in PBS prior to TrueBlack treatment may help minimize this.View more FAQs