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Is staining with Mix-n-Stain™ labeled antibodies as sensitive as staining with unlabeled primary and fluorescent secondary antibodies?

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Direct immunofluorescence detection can be less sensitive than indirect detection. You may need to use a higher concentration of antibody or higher gain settings to achieve similar staining intensity compared to indirect immunofluorescence staining. In our internal testing, indirect immunofluorescence staining results in about 3-fold signal amplification compared to direct immunofluorescence staining.

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