September 25, 2013

What are the fluorescence lifetimes of CF® Dyes?

By: Rhonda Sorensen

Lifetime data for several CF® Dyes are available, both for the free dye in buffer as well as for the corresponding CF® Dye conjugated secondary antibody. These are listed below.

Dye	τ (ns) /free acid in PBS pH 7.4, ε (ns)	τ (ns) /S.Ab§
CF®405S	3.88 ± 0.05	-
CF®488A	4.11 ± 0.05	1.705
CF®568	3.66 ± 0.05	1.539
CF®594	-	1.746
CF®633	3.39* (in water)	3*
CF®640R	2.38 ± 0.05	1.557
CF®647	1.07 ± 0.05	1.195
CF®680	1.23 ± 0.05	1.277
CF®680R	1.22 ± 0.05	1.6
CF®750	0.58 ± 0.05	0.636
CF®790	0.39 ± 0.05	0.54

Measurements were made on a Stellaris 8 STED FALCON microscope courtesy Leica Microsystems, Germany. § Fluorescence lifetime measurements of CF® Dye labeled anti-mouse secondary antibodies used for immunostaining microtubules in U2OS using mouse anti-tubulin (DM1a) and mounted in ProLong™ Diamond. *Lifetime data obtained via customer communication under different experimental conditions and imaging setup.