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What is the difference between CellBrite™ dyes and PKH dyes?

Biotium’s CellBrite™ Cytoplasmic Membrane Dyes are dye delivery solutions that can be added directly to normal culture media to uniformly label suspended or adherent cells in culture. The PKH dyes are structurally related dyes for cell membrane labeling. But unlike CellBrite™, labeling with PKH dyes requires multiple steps and subjects cells to an iso-osmotic mannitol loading medium that can negatively affect cell membrane integrity and viability.

CellBrite™ Fix dyes also feature rapid and simple labeling in isotonic buffer. Cells can be fixed and permeabilized after labeling with CellBrite™ Fix, unlike original CellBrite™ dyes or PKH dyes, which don’t tolerate detergent.

Category: CellBrite™ Cytoplasmic Membrane Stains

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