All three of these dyes can be excited by the 405 nm laser (or UV mercury lamp). They differ in their emission wavelengths. CF®405S has blue fluorescence emission at 431 nm, similar to AlexaFluor® 405, Cascade Blue®, and DyLight™ 405 (Thermo Scientific). CF®405M has longer wavelength blue fluorescence emission at 452 nm, similar to Horizon™ V450 (BD Biosciences) and Pacific Blue® (Thermo Scientific). CF®405L has orange fluorescence emission at 545 nm, similar to Pacific Orange® (Thermo Scientific). We recommend choosing the dye that best fits your instrument’s detection settings. See our CF® Dye Quick Reference Table and CF® Dye Selection Guide for more information on CF® dyes.View more FAQs
What is the difference between CF®405S, CF®405M, and CF®405L?
Apoptosis, Necrosis, and Cell Viability KitsCellBrite® & MemBrite® Membrane & Cell Surface StainsCF® DyesDNA Quantitation KitsDNAzure® Visible DNA Gel StainEvaGreen® Dye and Master MixesEverBrite™ Mounting Medium & CoverGrip™ Coverslip SealantExosome & EV StainingGelRed® and GelGreen® Nucleic Acid Gel StainsGelRed® and GelGreen® TroubleshootingIon Indicators & ChelatorsLuciferase AssaysMicrobiology: Dyes for bacteria and yeastMix-n-Stain™ Antibody Labeling KitsNucView® Caspase-3 Enzyme SubstratesOrganelle & Cytoskeleton StainsOther DNA/RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)