Our qPCR Master Mixes are all offered with optional ROX reference dye, which is used by some qPCR instruments to normalize the PCR fluorescence. We also include instructions for using no ROX, low ROX, or high ROX, depending on the instrument you are using. Based on instrument manufacturers’ recommendations, we recommend the following:
No ROX
BioRad: iCycler™, MyiQ™, MiQ™2, iQ™5, CFX-96 Touch™, CFX-384 Touch™, Chromo4™, MiniOpticon™
Qiagen: Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000
Eppendorf: Mastercycler® Realplex
Illumina: Eco™ RealTime PCR System
Cepheid: SmartCycler®
Roche: LightCycler® 480, LightCycler® 2.0
Low ROX
ABI: 7500, 7500 Fast, ViiA 7™, QuantStudio™
Stratagene: MX4000P, MX3000P, MX3005P
High ROX
ABI: 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne®, StepOne Plus®
View more FAQs