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JC-1 Mitochondrial Membrane Potential Detection Kit

JC-1 Mitochondrial Membrane Potential Detection Kit is used to measure mitochondrial membrane potential changes in cells.

Product Attributes

Apoptosis/viability marker

Mitochondrial potential

For live or fixed cells

For live/intact cells

Detection method/readout

Microplate reader (fluorescence), Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Colors

Green/Red

Size
Catalog #
price
Qty
Trial size (25 assays)
100 assays
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Product Description

The loss of mitochondrial membrane potential is a hallmark for apoptosis. It is an early event preceding phosphatidylserine externalization and coinciding with caspase activation (1,2). JC-1 Mitochondrial Membrane Potential Detection Kit is used to measure mitochondrial membrane potential changes in cells. In non-apoptotic cells, JC-1 accumulates as aggregates in the mitochondrial membranes, resulting in red fluorescence (590 nm). The brightness of red fluorescence is proportional to the potential and varies among different cell types. However, in apoptotic and necrotic cells, which have diminished mitochondrial membrane potential, JC-1 exists in the green fluorescent (529 nm) monomeric form (3-5). This kit provides a step-by-step protocol and ready-to-use reagents for performing assays for flow cytometry, fluorescence microscopy, or fluorometric microplate reader.

 

We also offer JC-1 iodide salt and JC-1 chloride salt.

References

1. J Ocular Pharmacol Therapeutics (2011) doi: 10.1089/jop.2010.0143

2. Breast Cancer Research 12, R49 (2010).

3. Breast Cancer Res. Treat (2010) doi: 10.1007/s10549-010-1085-7

4. J Immunol 184, 1111-1120 (2010).

5. Exp Hematol 31, 815 (2003).

6. Br J Haematol 108, 574 (2000).

7. J. Neurochem 70, 66 (1998).

8. Cytometry 29, 97 (1997).

9. FEBS Lett 411, 77 (1997).

10. Anal. Chem (2013) doi: 10.1021/ac3025812

 

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