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JC-1 Mitochondrial Membrane Potential Detection Kit

JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.

Product Attributes

Apoptosis/viability marker

Mitochondrial potential

For live or fixed cells

For live/intact cells

Detection method/readout

Microplate reader (fluorescence), Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Potential dependence

Mitochondrial potential-dependent

Indicator type

Ratiometric

Colors

Green/Red

Excitation/Emission

510/527 nm (monomer); 585/590 nm (aggregate)

Storage Conditions

Store at 2 to 8 °C, Protect dye component from light

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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100 assays
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Product Description

JC-1 Mitochondrial Membrane Potential Detection Kit is used to measure mitochondrial membrane potential changes in cells, a key indicator of cell viability.

Features

  • Rapid, simple measurement of mitochondrial membrane potential/cell viability
  • Healthy cells stain red & green, dead cells stain green
  • For fluorescence microscopy, flow cytometry, or fluorescence microplate reader

Spectral properties

  • Ex/Em (cytoplasm): 510/527 nm (green)
  • Ex/Em (polarized mitochondria): 585/590 nm (red)

Kit components

  • JC-1, 100X in DMSO
  • 10X Assay Buffer

The loss of mitochondrial membrane potential is a hallmark for apoptosis. It is an early event preceding phosphatidylserine externalization and coinciding with caspase activation.  In non-apoptotic cells, JC-1 accumulates as aggregates in the mitochondrial membranes, resulting in red fluorescence. The brightness of red fluorescence is proportional to the potential and varies among different cell types. However, in apoptotic and necrotic cells, which have diminished mitochondrial membrane potential, JC-1 exists in the green fluorescent monomeric form. This kit provides a step-by-step protocol and ready-to-use reagents for performing assays for flow cytometry, fluorescence microscopy, or fluorescence microplate reader.

We also offer JC-1 iodide salt and JC-1 chloride salt.

References

 

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