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JC-1 Mitochondrial Membrane Potential Detection Kit

JC-1 Mitochondrial Membrane Potential Detection Kit is used to measure mitochondrial membrane potential changes in cells.

Product Attributes

Apoptosis/viability marker

Mitochondrial potential

For live or fixed cells

For live/intact cells

Detection method/readout

Microplate reader (fluorescence), Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay

Potential dependence

Mitochondrial potential-dependent

Indicator type





510/527 nm (monomer); 585/590 nm (aggregate)

Catalog #
Trial size (25 assays)
100 assays
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Product Description

The loss of mitochondrial membrane potential is a hallmark for apoptosis. It is an early event preceding phosphatidylserine externalization and coinciding with caspase activation (1,2). JC-1 Mitochondrial Membrane Potential Detection Kit is used to measure mitochondrial membrane potential changes in cells. In non-apoptotic cells, JC-1 accumulates as aggregates in the mitochondrial membranes, resulting in red fluorescence. The brightness of red fluorescence is proportional to the potential and varies among different cell types. However, in apoptotic and necrotic cells, which have diminished mitochondrial membrane potential, JC-1 exists in the green fluorescent monomeric form (3-5). This kit provides a step-by-step protocol and ready-to-use reagents for performing assays for flow cytometry, fluorescence microscopy, or fluorometric microplate reader.

Spectral properties:

  • Ex/Em (cytoplasm): 510/527 nm (green)
  • Ex/Em (polarized mitochondria): 585/590 nm (red)

Kit components:

  • JC-1, 100X in DMSO
  • 10X Assay Buffer

We also offer JC-1 iodide salt and JC-1 chloride salt.


1. J Ocular Pharmacol Therapeutics (2011) doi: 10.1089/jop.2010.0143

2. Breast Cancer Research 12, R49 (2010).

3. Breast Cancer Res. Treat (2010) doi: 10.1007/s10549-010-1085-7

4. J Immunol 184, 1111-1120 (2010).

5. Exp Hematol 31, 815 (2003).

6. Br J Haematol 108, 574 (2000).

7. J. Neurochem 70, 66 (1998).

8. Cytometry 29, 97 (1997).

9. FEBS Lett 411, 77 (1997).

10. Anal. Chem (2013) doi: 10.1021/ac3025812


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