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MitoView™ Fix 640

Far-red, mitochondrial stain with excellent tolerance for fixation and permeabilization after staining.

Product Attributes

Cellular localization

Mitochondria

Assay type/options

Long term staining (24-72h), No-wash staining

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining

Colors

Far-red

Excitation/Emission

648/670 nm

Storage Conditions

Store at 2 to 8 °C or below, Protect from light

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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50 ug
10 x 50 ug
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Product Description

MitoView™ Fix 640 is far-red mitochondrial stain. The cell membrane-permeant dye rapidly accumulates in the mitochondria of live cells for no-wash, live cell staining. The dye has a reactive group that can covalently attach to proteins inside the mitochondria, so that staining is well-retained after fixation, permeabilization, and subsequent immunofluorescence staining.

  • Stain mitochondria in live cells for subsequent fixation
  • Well-retained after PFA or MeOH fixation, permeabilization, and IF staining
  • Bright, photostable, and non-toxic
  • Cells retain specific staining in culture for days
  • Far-red fluorescence for the Cy®5 channel (Ex/Em 648/670 nm)
  • Better specificity after fixation compared to MitoTracker® Deep-Red

MitoView™ Fix is not recommended for staining cells that are already fixed, because staining is non-specific in fixed cells. MitoView™ Green dye can be used for fixed cell staining, but for best results with fixed cells, we recommend using one of our mitochondrial marker antibody conjugates.

We also offer original MitoView™ Dyes in a variety of colors, including membrane potential-dependent MitoView™ 633, in addition to a selection of classic mitochondrial dyes. See our Cellular Stains for more stains for mitochondria, nucleus, cell surface, lysosomes, and other organelles.

Cy dye is a registered trademark of GE Healthcare; MitoTracker is a registered trademark of Thermo Fisher Scientific.
 

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