5-CFDA (5-Carboxyfluorescein diacetate, single isomer)
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).
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Product Description
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).
- Off-white solid soluble in DMSO
- Store desiccated at -20°C
- C25H16O9
- MW: 460
- [79955-27-4]
References
1. J Cell Sci 98, 343(1991).
2. Nature 295, 524 (1982).
3. J Immunol Meth 100, 261 (1987).
4. J Immunol Meth 33, 33 (1980).
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6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein.
5-(and-6)-Carboxy-2′,7′- dichlorofluorescein
5-(and-6)-carboxy-2',7'-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment.
CDCFDA
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2',7'-dichlorofluorescein (51015) by intracellular esterases.
5-Carboxy-2′,7′-dichlorofluorescein (single isomer)
This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label.
5-CFDA (5-Carboxyfluorescein diacetate, single isomer)
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).