AKT1 Monoclonal Mouse Antibody (AKT1/2552)
This antibody recognizes a protein of 62 kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBbeta or RacPK-beta) and Akt3 (also designated PKB-gamma or thymoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-beta tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Catalog number key for antibody number 2552, Anti-AKT1 (AKT1/2552)
Catalog Number Key for Primary Antibody Conjugates
|Antibody # prefix||Conjugation||Ex/Em (nm)||Laser line||Detection channel||Dye Features|
|BNC04||CF®405S||404/431||405||DAPI (microscopy), AF405||CF®405S Features|
|BNC88||CF®488A||490/515||488||GFP, FITC||CF®488A Features|
|BNC68||CF®568||562/583||532, 561||RFP, TRITC||CF®568 Features|
|BNC94||CF®594||593/614||561||Texas Red®||CF®594 Features|