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ALK / CD246 Recombinant Monoclonal Rabbit Antibody (ALK/3218R)

Product Attributes

research-areas

Cancer, Neuroscience, Signal transduction

Antibody number

#3218

Antibody reactivity (target)

ALK, CD246

Antibody type

Primary

Host species

Rabbit

Clonality

Recombinant Monoclonal

Clone

ALK/3218R

Isotype

IgG

Molecular weight

80 kDa (hybrid); 200 kDa (wild type)

Synonyms

ALK Tyrosine Kinase Receptor; Anaplastic Lymphoma Kinase; ALK/NPM1 fusion gene

Human gene symbol

ALK

Entrez gene ID

238

SwissProt

Q9UM73

Unigene

654469

Immunogen

Recombinant human ALK protein fragment (aa200-335)

Antibody target cellular localization

Cytoplasmic, Nucleus

Verified antibody applications

IHC (FFPE) (verified)

Species reactivity

Human

Positive control

Anaplastic Large Cell Lymphoma

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Tumor expression

Leukemia/lymphoma

Conjugation
Note: Please be advised Biotium will no longer be selling primary antibodies conjugated to R-PE, APC, PerCP, AP, or HRP as of 4/18/22. However, certain protein-labeled primary antibodies will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with these conjugates. We apologize for the inconvenience.
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Product Description

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200 kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes). ALK has been found to be rearranged, mutated, or amplified in a series of tumors. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation, encodes part of the nucleolar phosphoprotein nucleophosmin (NPM), joined to the entire cytoplasmic portion of ALK. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80 kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein, producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 3218, ALK / CD246 Recombinant Monoclonal Rabbit Antibody (ALK/3218R)

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC05CF®405M408/452405DAPI (microscopy),
Pacific Blue™
CF®405M Features
BNC06CF®405L395/545405Pacific Orange™CF®405L Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC14CF®514516/548488GFP, FITCCF®514 Features
BNC43CF®543541/560532RFP, TRITCCF®543 Features
BNC55CF®555555/565532, 561RFP, TRITCCF®555 Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC60CF®660C667/685633-640Cy®5CF®660C Features
BNC61CF®660R663/682633-640Cy®5CF®660R Features
BNC80CF®680681/698633-640Cy®5.5, IR®700CF®680 Features
BNC81CF®680R680/701633-640Cy®5.5, IR®700CF®680R Features
BNC00CF®700695/720633-640AF700CF®700 Features
BNC70CF®770770/797785, 808IR®800CF®770 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
 

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