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Annexin V Conjugates

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

Product Attributes

Storage Conditions

Store at 2 to 8 °C, Do not freeze, Protect fluorescent conjugates from light

Apoptosis/viability marker

Phosphatidylserine/Annexin V

For live or fixed cells

For live/intact cells

Assay type/options

Short term staining (<24h), Endpoint assay, No-wash staining, Real-time imaging, Homogeneous assay

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Format

Fluorescent dye & biotin conjugates: Supplied in 10 mM Tris pH 7.5, 1 mM EDTA, 30 mM NaCl, 1 mg/mL BSA, 0.1% sodium azide
R-PE & APC conjugates: Supplied in PBS, 5 mg/mL BSA, 0.05% sodium azide

Colors

Blue, Green, Orange, Red, Far-red

Fixation options

Fix after staining (formaldehyde)

Product origin

Annexin V (human); recombinant, produced in E. coli

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Technology

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

  • Detect apoptotic cells with fast, reliable detection of phosphatidylserine externalization
  • Conjugates with wide choice of CF® dye colors, plus biotin, R-PE, APC, and other fluorescent dyes
  • Biotium’s CF® dyes have superior brightness, photostability, and are water-soluble
  • Can be used for fluorescence microscopy, live cell imaging, and flow cytometry

Annexin V conjugates are provided as 50 ug/mL solutions in 10 mM Tris, 1 mM EDTA, 30 mM NaCl, 1 mg/mL bovine serum albumin, 0.1% sodium azide, pH 7.5.

Annexin V Conjugates
Click to view timelapse.
Imaging of apoptosis in staurosporine-treated HeLa cells stained with CF®594 Annexin V (red, apoptotic cell surface probe) and Oxazole Yellow (green, dead cell nuclear stain). All nuclei are stained blue with Hoechst.

Detect Apoptosis

We also offer Annexin V Conjugates, Azide-Free and Lyophilized in colors ranging from UV to far-red for live cell staining, and Near-IR CF® Dye Annexin V (also lyophilized and preservative-free), compatible with small animal NIR in vivo imaging. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Annexin V Conjugates

ProductEx/EmConcentrationSizeCatalog No.
Biotin Annexin VN/A50 ug/mL0.5 mLPurchase 29013
CF®350 Annexin V 347/448 nm50 ug/mL0.5 mLPurchase 29012
CF®405M Annexin V 408/452 nm50 ug/mL0.5 mLPurchase 29009
CF®450 Annexin V 405/460 nm50 ug/mL0.5 mLPurchase 29083
CF®488A Annexin V 490/515 nm50 ug/mL0.5 mLPurchase 29005
CF®555 Annexin V 555/565 nm50 ug/mL0.5 mLPurchase 29004
CF®568 Annexin V 562/583 nm50 ug/mL0.5 mLPurchase 29010
CF®583R Annexin V 583/606 nm50 ug/mL0.5 mLPurchase 29085
CF®594 Annexin V 593/614 nm50 ug/mL0.5 mLPurchase 29011
CF®633 Annexin V 630/650 nm50 ug/mL0.5 mLPurchase 29008
CF®640R Annexin V 642/662 nm50 ug/mL0.5 mLPurchase 29014
CF®647 Annexin V 650/665 nm50 ug/mL0.5 mLPurchase 29003
CF®660R Annexin V 663/682 nm50 ug/mL0.5 mLPurchase 29069
R-PE Annexin V 496, 546,
565/578 nm
5 uL/test100 uLPurchase 29045-100uL
500 uLPurchase 29045-500uL
APC Annexin V 650/660 nm5 uL/test100 uLPurchase 29057-100uL
500 uLPurchase 29057-500uL
FITC Annexin V 496/516 nm50 ug/mL0.5 mLPurchase 29001
5X Annexin V Binding BufferN/A5X15 mLPurchase 99902

 

CF is a registered trademark of Biotium, Inc. Alexa Fluor, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

Download a list of CF® dye Annexin V references.

 

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