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Annexin V Near IR CF® Dye Conjugates

Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry, Near-IR detection, In vivo near-IR imaging

Assay type/options

Long term staining (24-72h), Endpoint assay, No-wash staining, Real-time imaging, Homogeneous assay

Fixation options

Fix after staining (formaldehyde)

Colors

Near-infrared

Product origin

Annexin V (human); recombinant, produced in E. coli

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Conjugation
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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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25 ug
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Product Description

Annexin V is a 35-36 kDa protein that has a high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasmic membrane, making itself available for Annexin V binding. Near-IR CF® Dye Annexin V does not contain azide or other potentially toxic materials, and thus is suitable for in vivo imaging.

  • Detect apoptotic cells with fast, reliable detection of phosphatidylserine externalization
  • No azide or other potentially toxic materials or preservatives, suitable for for in vivo imaging
  • Choice of 7 near-IR fluorescent CF® dye colors
  • Biotium’s CF® dyes are far more photostable and brighter than other similar near-IR dyes
  • Can be used for fluorescence microscopy, live cell imaging, and flow cytometry

Superior CF® Dyes

CF® dyes are far more photostable and brighter than other similar near-IR dyes, such as Alexa Fluor® 700,  Alexa Fluor® 750, Cy®7 and IRDye® 800. Learn more about Near-IR CF® Dyes in the Near-IR Dye Flyer. Learn more about CF® Dyes.

Detect Apoptosis with Annexin V

We also offer Annexin V Conjugates, Azide-Free, and Lyophilized in colors ranging from UV to far-red for live cell staining. Annexin V Conjugate solutions (with azide) also are available with a large selection of CF® Dyes, biotin, R-PE, APC, and other labels. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.

Annexin V Near-IR Conjugates

ProductConjugationEx/EmSizeCatalog No.Purchase
CF®680 Annexin V CF®680681/698 nm25 ug29007Purchase 29007
CF®680R Annexin V CF®680R680/701 nm25 ug29070Purchase 29070
CF®700 Annexin V CF®700699/737 nm25 ug29082Purchase 29082
CF®750 Annexin V CF®750755/777 nm25 ug29006Purchase 29006
CF®770 Annexin V CF®770770/797 nm25 ug29046Purchase 29046
CF®790 Annexin V CF®790784/806 nm25 ug29047Purchase 29047
CF®800 Annexin V CF®800797/816 nm25 ug29078Purchase 29078
CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

Download a list of CF® dye Annexin V references.

 

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