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Annexin V Near IR CF Dye Conjugates

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available labeled with our superior near-IR fluorescent dyes.

Apoptotic Jurkat cell stained with NucView 488 (green) and CF647 Annexin V (magenta).
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Catalog #

29006, 29007, 29046, 29047, - 29078View allHide

Size

25 ug

Conjugation

CF680, CF750, CF770, CF790, CF800

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Product Description

CF™ dye Annexin V conjugate is Annexin V labeled with our superior near-IR fluorescent dye. Annexin V is a 35-36 kDa protein that has a high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasmic membrane, making itself available for Annexin V binding.

Near-IR fluorescent dyes available for this product are: CF750, CF680, CF770, CF790 and CF800.

These CF™ dyes are far more photostable and brighter than other similar near-IR dyes, such as Alexa Fluor® 750, Cy®7 and IRDye® 800 (download the Near-IR Dye Flyer for more information). CF™ dye Annexin V does not contain azide or other potentially toxic materials, and thus is suitable for in vivo imaging.

For more information, download our Apoptosis, Necrosis and Cell Viability Assays Brochure, CF™ Dye Selection Guide, and Flow Cytometry Products Brochure.

References

Download a list of CF dye references.

 

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    TUNEL Assay Apoptosis Detection Kit

    The CF™ dye TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.

    Live-or-Dye Fixable Viability Staining Kits

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Support & Faq

CF™ Dyes

See our CF™ Dye Quick Reference Table for a list of dyes and summary of their features. Our CF™ Dye Selection Guide has more detailed information on each CF™ dye, and ordering information for our various CF™ dye product lines. You can download CF™ dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF™ Dyes

← FAQs

CF initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF dyes based on cyanine dye structures. Since then, our CF dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF™ Dyes

← FAQs

The exact chemical structures of CF dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF dye invention that makes CF dyes superior to other commercial dyes.

Category: CF™ Dyes

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Apoptosis, Necrosis, and Cell Viability Kits

MTT and XTT are colorimetric based assays, while resazurin can be measured using colorimetric or fluorescence detection. MTT is not a soluble product, so the cells must be lysed to solubilize the formazan salt before absorbance can be measured. XTT and resazurin do not require cell lysis, allowing kinetic monitoring of the same samples at different timepoints.

Category: Apoptosis, Necrosis, and Cell Viability Kits

← FAQs

Check to make sure your cell densities are in the linear range of the cell viability assay. Too few cells may fall below the limit of detection, and too many cells may saturate the OD. Perform an initial experiment to check the best linear range for your cell type and experimental set-up. You may need to vary the cell density assay incubation time. The kit protocols provide general guidelines and may need to be optimized for your experimental system.

Category: Apoptosis, Necrosis, and Cell Viability Kits

← FAQs

Resazurin is reduced by cells to the fluorescent product resorufin. Resorufin can be reduced further to a non-fluorescent compound. Therefore the densest wells may have lowest fluorescence due to over-reduction of the substrate. Please see the product information sheet for more details. The kit protocol provides general guidelines and may need to be optimized empirically for your experimental system. You may need to vary cell density or assay incubation time to ensure that your samples fall in the linear range of the kit.

Category: Apoptosis, Necrosis, and Cell Viability Kits

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.