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Annexin V CF® Dye Conjugates, Azide-Free, Lyophilized

Preservative-free Annexin V conjugates are compatible with real-time staining of apoptotic cells for live cell imaging, fluorescence microscopy, or flow cytometry.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Assay type/options

Endpoint assay, Homogeneous assay, Long term staining (24-72h), No-wash staining, Real-time imaging


Blue, Green, Red, Far-red

Fixation options

Fix after staining (formaldehyde)

Product origin

Annexin V (human); recombinant, produced in E. coli

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Catalog #
5 ug
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Product Description

CF® Dye Annexin V Conjugates can be used to stain the surface of apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

  • Azide-free for no-wash, real-time live cell imaging in culture medium
  • Fast & simple detection of phosphatidylserine on apoptotic cells
  • Choice of 11 CF® dye colors with superior brightness & photostability
  • For real-time live cell imaging, fluorescence microscopy, or flow cytometry

Preservative Free for Real-Time, Live Cell Imaging

Annexin V conjugates typically are supplied as stock solutions with azide as a preservative for end-point staining assays in Annexin V binding buffer. Our azide-free CF® Dye Annexin V Conjugates are supplied as lyophilized solids with no azide or other preservatives that might be incompatible with live cell imaging. After reconstitution in buffer, the conjugates can be added to cell culture medium for no-wash, real-time live cell imaging. Our Mini Syringe Filters are convenient for small volume sterile filtration of azide-free Annexin V stock solutions or other aqueous solutions for use in cell culture.

See Annexin V staining in real time:

Annexin V Conjugates
Click to view timelapse.
Imaging of apoptosis in staurosporine-treated HeLa cells stained with CF®594 Annexin V (red, apoptotic cell surface probe) and Oxazole Yellow (green, dead cell nuclear stain). All nuclei are stained blue with Hoechst.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

More Apoptosis Assays

We also offer Near-IR CF® Dye Annexin V Conjugates, preservative-free and lyophilized, compatible with small animal in vivo imaging. Annexin V Conjugate solutions (with azide) also are available with a large selection of CF® Dyes, biotin, R-PE, APC, and other labels. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.

CF® Dye Annexin V Conjugates, Azide-Free, Lyophilized

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm5 ug29012-5ugCF®350 Features
CF®405M408/452 nm5 ug29009-5ugCF®405M Features
CF®450450/538 nm5 ug29083R-5ugCF®450 Features
CF®488A490/515 nm5 ug 29005R-5ugCF®488A Features
CF®555555/565 nm5 ug29004R-5ugCF®555 Features
CF®568562/583 nm5 ug29010R-5ugCF®568 Features
CF®583R586/609 nm5 ug29085R-5ugCF®583R Features
CF®594593/614 nm5 ug29011R-5ugCF®594 Features
CF®633630/650 nm5 ug29008R-5ugCF®633 Features
CF®640R642/662 nm5 ug29014R-5ugCF®640R Features
CF®647650/665 nm5 ug29003R-5ugCF®647 Features
CF®660R663/682 nm5 ug29069R-5ugCF®660R Features
CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.


Download a list of CF® dye Annexin V references.


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