Annexin V CF® Dye Conjugates, Azide-Free, Lyophilized
Preservative-free Annexin V conjugates are compatible with real-time staining of apoptotic cells for live cell imaging, fluorescence microscopy, or flow cytometry.
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Product Description
CF® Dye Annexin V Conjugates can be used to stain the surface of apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.
- Azide-free for no-wash, real-time live cell imaging in culture medium
- Fast & simple detection of phosphatidylserine on apoptotic cells
- Choice of 11 CF® dye colors with superior brightness & photostability
- For real-time live cell imaging, fluorescence microscopy, or flow cytometry
Preservative Free for Real-Time, Live Cell Imaging
Annexin V conjugates typically are supplied as stock solutions with azide as a preservative for end-point staining assays in Annexin V binding buffer. Our azide-free CF® Dye Annexin V Conjugates are supplied as lyophilized solids with no azide or other preservatives that might be incompatible with live cell imaging. After reconstitution in buffer, the conjugates can be added to cell culture medium for no-wash, real-time live cell imaging. Our Mini Syringe Filters are convenient for small volume sterile filtration of azide-free Annexin V stock solutions or other aqueous solutions for use in cell culture.
See Annexin V staining in real time:

Imaging of apoptosis in staurosporine-treated HeLa cells stained with CF®594 Annexin V (red, apoptotic cell surface probe) and Oxazole Yellow (green, dead cell nuclear stain). All nuclei are stained blue with Hoechst.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
More Apoptosis Assays
We also offer Near-IR CF® Dye Annexin V Conjugates, preservative-free and lyophilized, compatible with small animal in vivo imaging. Annexin V Conjugate solutions (with azide) also are available with a large selection of CF® Dyes, biotin, R-PE, APC, and other labels. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.
CF® Dye Annexin V Conjugates, Azide-Free, Lyophilized
Product | Ex/Em | Catalog No. | Size | Purchase |
---|---|---|---|---|
CF®350 Annexin V, Azide-Free, Lyophilized | 347/448 nm | 29012R-5ug | 5 ug | Purchase 29012R-5ug |
CF®405M Annexin V, Azide-Free, Lyophilized | 408/452 nm | 29009R-5ug | 5 ug | Purchase 29009R-5ug |
CF®450 Annexin V, Azide-Free, Lyophilized | 450/538 nm | 29083R-5ug | 5 ug | Purchase 29083R-5ug |
CF®488A Annexin V, Azide-Free, Lyophilized | 490/515 nm | 29005R-5ug | 5 ug | Purchase 29005R-5ug |
CF®555 Annexin V, Azide-Free, Lyophilized | 555/565 nm | 29004R-5ug | 5 ug | Purchase 29004R-5ug |
CF®568 Annexin V, Azide-Free, Lyophilized | 562/583 nm | 29010R-5ug | 5 ug | Purchase 29010R-5ug |
CF®583R Annexin V, Azide-Free, Lyophilized | 586/609 nm | 29085R-5ug | 5 ug | Purchase 29085R-5ug |
CF®594 Annexin V, Azide-Free, Lyophilized | 593/614 nm | 29011R-5ug | 5 ug | Purchase 29011R-5ug |
CF®633 Annexin V, Azide-Free, Lyophilized | 630/650 nm | 29008R-5ug | 5 ug | Purchase 29008R-5ug |
CF®640R Annexin V, Azide-Free, Lyophilized | 642/662 nm | 29014R-5ug | 5 ug | Purchase 29014R-5ug |
CF®647 Annexin V, Azide-Free, Lyophilized | 650/665 nm | 29003R-5ug | 5 ug | Purchase 29003R-5ug |
CF®660R Annexin V, Azide-Free, Lyophilized | 663/682 nm | 29069R-5ug | 5 ug | Purchase 29069R-5ug |
References
Download a list of CF® dye Annexin V references.