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Annexin V CF® Dye Conjugates, Azide-Free, Lyophilized

Preservative-free Annexin V conjugates are compatible with real-time staining of apoptotic cells for live cell imaging, fluorescence microscopy, or flow cytometry.

Product Attributes

Apoptosis/viability marker

Phosphatidylserine/Annexin V

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Assay type/options

Long term staining (24-72h), Endpoint assay, No-wash staining, Real-time imaging, Homogeneous assay

Colors

Blue, Green, Red, Far-red

Fixation options

Fix after staining (formaldehyde)

Product origin

Annexin V (human); recombinant, produced in E. coli

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Conjugation
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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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5 ug
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Product Description

CF® Dye Annexin V Conjugates can be used to stain the surface of apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

  • Azide-free for no-wash, real-time live cell imaging in culture medium
  • Fast & simple detection of phosphatidylserine on apoptotic cells
  • Choice of 11 CF® dye colors with superior brightness & photostability
  • For real-time live cell imaging, fluorescence microscopy, or flow cytometry

Preservative Free for Real-Time, Live Cell Imaging

Annexin V conjugates typically are supplied as stock solutions with azide as a preservative for end-point staining assays in Annexin V binding buffer. Our azide-free CF® Dye Annexin V Conjugates are supplied as lyophilized solids with no azide or other preservatives that might be incompatible with live cell imaging. After reconstitution in buffer, the conjugates can be added to cell culture medium for no-wash, real-time live cell imaging. Our Mini Syringe Filters are convenient for small volume sterile filtration of azide-free Annexin V stock solutions or other aqueous solutions for use in cell culture.

See Annexin V staining in real time:

Annexin V Conjugates
Click to view timelapse.
Imaging of apoptosis in staurosporine-treated HeLa cells stained with CF®594 Annexin V (red, apoptotic cell surface probe) and Oxazole Yellow (green, dead cell nuclear stain). All nuclei are stained blue with Hoechst.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

More Apoptosis Assays

We also offer Near-IR CF® Dye Annexin V Conjugates, preservative-free and lyophilized, compatible with small animal in vivo imaging. Annexin V Conjugate solutions (with azide) also are available with a large selection of CF® Dyes, biotin, R-PE, APC, and other labels. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.

CF® Dye Annexin V Conjugates, Azide-Free, Lyophilized

ProductEx/EmCatalog No.SizePurchase
CF®350 Annexin V, Azide-Free, Lyophilized347/448 nm29012R-5ug5 ugPurchase 29012R-5ug
CF®405M Annexin V, Azide-Free, Lyophilized 408/452 nm29009R-5ug5 ugPurchase 29009R-5ug
CF®450 Annexin V, Azide-Free, Lyophilized 450/538 nm29083R-5ug5 ugPurchase 29083R-5ug
CF®488A Annexin V, Azide-Free, Lyophilized 490/515 nm 29005R-5ug5 ugPurchase 29005R-5ug
CF®555 Annexin V, Azide-Free, Lyophilized 555/565 nm29004R-5ug5 ugPurchase 29004R-5ug
CF®568 Annexin V, Azide-Free, Lyophilized 562/583 nm29010R-5ug5 ugPurchase 29010R-5ug
CF®583R Annexin V, Azide-Free, Lyophilized 586/609 nm29085-5ug5 ugPurchase 29085R-5ug
CF®594 Annexin V, Azide-Free, Lyophilized 593/614 nm29011R-5ug5 ugPurchase 29011R-5ug
CF®633 Annexin V, Azide-Free, Lyophilized 630/650 nm29008R-5ug5 ugPurchase 29008R-5ug
CF®640R Annexin V, Azide-Free, Lyophilized 642/662 nm29014R-5ug5 ugPurchase 29014R-5ug
CF®647 Annexin V, Azide-Free, Lyophilized 650/665 nm29003R-5ug5 ugPurchase 29003R-5ug
CF®660R Annexin V, Azide-Free, Lyophilized 663/682 nm29069R-5ug5 ugPurchase 29069R-5ug
CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

Download a list of CF® dye Annexin V references.

 

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