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CD16 Mouse Monoclonal Antibody (3G8) – Biotium Choice

CD16 (clone 3G8) is a validated mouse monoclonal antibody that recognizes CD16 (also known as FC gamma receptor III), the low-affnitity receptor for IgG. The antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance.

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Product Attributes

Antibody number

P011

Antibody reactivity (target)

CD16

Biotium Choice Antibody

Primary

Antibody type

Biotium Choice Flow Antibodies, Primary

Host species

Mouse

Clonality

Monoclonal

Clone

3G8

Isotype

IgG1, kappa

Molecular weight

29 kDa (predicted); 50-65 kDa (observed)

Synonyms

FCG3A, Fc gamma receptor 3

Human gene symbol

CD16

Entrez gene ID

2214

SwissProt

P08637

Unigene

372679

Immunogen

Human

Antibody target cellular localization

Plasma membrane

Verified antibody applications

Flow (surface) (verified)

Species reactivity

Human

Antibody application notes

Recommended concentration for flow cytometry: 5 uL per million cells/0.1 mL

Positive control

Human peripheral blood mononuclear cells (PBMCs)

Shipping condition

Room temperature

Storage Conditions

Protect fluorescent conjugates from light

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Volume per assay

5 uL/test

Antibody/conjugate formulation

Conjugates: PBS/0.1% BSA/0.05% azide

Antibody research areas

Immunology

Cell/tissue expression

Lymphocytes

Conjugation
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Product Description

CD16 (clone 3G8) is a mouse monoclonal antibody that recognizes IgG receptor CD16, also known as FC gamma receptor III. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. The antibody is available conjugated to CF® Dyes and Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.

This antibody recognizes CD16 (Fc gamma receptor III), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gamma receptor IIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gamma receptor IIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody. View our full catalog of Biotium Choice antibodies, optimized and validated for the best results in flow cytometry.

CD16 Monoclonal Mouse Antibody (3G8) - Biotium Choice

ConjugationEx/EmConc.SizeCatalog No.Dye Features
CF®405M416/452 nm100 ug/mL25 tests (125 uL)P011-405M-125CF®405M Features
100 tests (500 uL)P011-405M-500
CF®488A490/516 nm50 ug/mL25 tests (125 uL)P011-488A-125CF®488A Features
100 tests (500 uL)P011-488A-500
CF®568562/584 nm50 ug/mL25 tests (125 uL)P011-568-125CF®568 Features
100 tests (500 uL)P011-568-500
RPE-Astral™616496, 546, 566/617 nm200 ug/mL25 tests (125 uL)P011-R616-125
100 tests (500 uL)P011-R616-500
CF®647Plus652/668 nm25 ug/mL25 tests (125 uL)P011-647P-125
100 tests (500 uL)P011-647P-500
CF®700696/721 nm100 ug/mL25 tests (125 uL)P011-700-125CF®700 Features
100 tests (500 uL)P011-700-500
APC-Astral™813633, 638/813 nm100 ug/mL25 tests (125 uL)P011-A813-125
100 tests (500 uL)P011-A813-500
R-PE496, 546, 565/576 nm100 ug/mL25 tests (125 uL)P011-RPE-125
100 tests (500 uL)P011-RPE-500