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CD19 Monoclonal Mouse Antibody (SJ25C1) – Biotium Choice

CD19 (clone SJ25C1) is a validated mouse monoclonal antibody that recognizes CD19, a transmembrane glycoprotein in B cells. The antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance.

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Product Attributes

Antibody number


Antibody reactivity (target)


Biotium Choice Antibody


Antibody type

Biotium Choice Flow Antibodies, Primary

Host species







IgG1, kappa

Molecular weight

95 kDa


B-Lymphocytes Surface Antigen B4; T-cell Surface Antigen Leu-12; CVID3; B4

Human gene symbol


Entrez gene ID






Antibody target cellular localization

Plasma membrane

Verified antibody applications

Flow (surface) (verified)

Species reactivity


Antibody application notes

Recommended concentration for flow cytometry: 5 uL per million cells/0.1 mL

Positive control

Human peripheral blood mononuclear cells (PBMCs)

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: PBS/0.1% BSA/0.05% azide

Antibody research areas


Cell/tissue expression

B-cells, Lymphocytes

Volume per assay

5 uL/test

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Catalog #
25 tests
100 tests
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Product Description

CD19 (clone SJ25C1) is a mouse monoclonal antibody that recognizes the transmembrane glycoprotein CD19. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. The antibody is available conjugated to CF® Dyes and Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.

CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody. View our full catalog of Biotium Choice antibodies, optimized and validated for the best results in flow cytometry.

CD19 Monoclonal Mouse Antibody (SJ25C1) - Biotium Choice

ConjugationEx/EmConc.SizeCatalog No.Dye Features
CF®405M416/452 nm100 ug/mL25 tests (125 uL)P010-405M-125CF®405M Features
100 tests (500 uL)P010-405M-500
CF®488A490/516 nm100 ug/mL25 tests (125 uL)P010-488A-125CF®488A Features
100 tests (500 uL)P010-488A-500
CF®575575/601 nm200 ug/mL25 tests (125 uL)P010-575-125
100 tests (500 uL)P010-575-500
RPE-Astral™616496, 546, 566/617 nm50 ug/mL25 tests (125 uL)P010-R616-125
100 tests (500 uL)P010-R616-500
CF®647Plus652/668 nm25 ug/mL25 tests (125 uL)P010-647P-125
100 tests (500 uL)P010-647P-500
CF®700696/721 nm100 ug/mL25 tests (125 uL)P010-700-125CF®700 Features
100 tests (500 uL)P010-700-500
APC-Astral™813633, 638/813 nm50 ug/mL25 tests (125 uL)P010-A813-125
100 tests (500 uL)P010-A813-500
R-PE496, 546, 565/576 nm50 ug/mL25 tests (125 uL)P010-RPE-125
100 tests (500 uL)P010-RPE-500