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CD8a Monoclonal Mouse Antibody (SK1) – Biotium Choice

CD8a (clone SK1) is a validated mouse monoclonal antibody that recognizes CD8a, a transmembrane glycoprotein and co-receptor found in T cells. The antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance.

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Product Attributes

Antibody number


Antibody reactivity (target)


Biotium Choice Antibody


Antibody type

Biotium Choice Flow Antibodies, Primary

Host species







IgG1, kappa

Molecular weight

32-34 kDa (alpha chain)


CD8, Leu2, MAL, p32

Human gene symbol


Entrez gene ID







Human peripheral blood T cells

Antibody target cellular localization

Plasma membrane

Verified antibody applications

Flow (surface) (verified)

Species reactivity


Antibody application notes

Recommended concentration for flow cytometry: 5 uL per million cells/0.1 mL

Positive control

Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: PBS/0.1% BSA/0.05% azide

Antibody research areas


Volume per assay

5 uL/test

Cell/tissue expression

Lymphocytes, T-cells

Tumor expression


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Catalog #
25 tests
100 tests
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Product Description

CD8a (clone SK1) is a mouse monoclonal antibody derived from the CD8 alpha chain. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. The antibody is available conjugated to CF® Dyes and Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.

CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4 /CD8-. Anaplastic large cell lymphoma is usually CD4 and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody. View our full catalog of Biotium Choice antibodies, optimized and validated for the best results in flow cytometry.

CD8a Monoclonal Mouse Antibody (SK1) - Biotium Choice

ConjugationEx/EmConc.SizeCatalog No.
CF®405S411/431 nm200 ug/mL25 tests (125 uL)P009-405S-125CF®405S Features
100 tests (500 uL)P009-405S-500
CF®405M416/452 nm100 ug/mL25 tests (125 uL)P009-405M-125CF®405M Features
100 tests (500 uL)P009-405M-500
CF®405L413/547 nm300 ug/mL25 tests (125 uL)P009-405L-125CF®405L Features
100 tests (500 uL)P009-405L-500
CF®488A490/516 nm100 ug/mL25 tests (125 uL)P009-488A-125CF®488A Features
100 tests (500 uL)P009-488A-500
CF®503R503/532 nm50 ug/mL25 tests (125 uL)P009-503R-125CF®503R Features
100 tests (500 uL)P009-503R-500
CF®514516/549 nm100 ug/mL25 tests (125 uL)P009-514-125CF®514 Features
100 tests (500 uL)P009-514-500
CF®568562/584 nm100 ug/mL25 tests (125 uL)P009-568-125CF®568 Features
100 tests (500 uL)P009-568-500
CF®575575/601 nm200 ug/mL25 tests (125 uL)P009-575-125
100 tests (500 uL)P009-575-500
RPE-Astral™616496, 546, 566/617 nm200 ug/mL25 tests (125 uL)P009-R616-125
100 tests (500 uL)P009-568-500
CF®647Plus652/668 nm50 ug/mL25 tests (125 uL)P009-647P-125
100 tests (500 uL)P009-647P-500
CF®660C667/685 nm100 ug/mL25 tests (125 uL)P009-660C-125CF®660C Features
100 tests (500 uL)P009-660C-500
CF®700696/721 nm100 ug/mL25 tests (125 uL)P009-700-125CF®700 Features
100 tests (500 uL)P009-700-500
APC-Astral™813633, 638/813 nm50 ug/mL25 tests (125 uL)P009-A813-125
100 tests (500 uL)P009-A813-500
R-PE496, 546, 565/576 nm60 ug/mL25 tests (125 uL)P009-RPE-125
100 tests (500 uL)P009-RPE-500
APC651/660 nm50 ug/mL25 tests (125 uL)P009-APC-125
100 tests (500 uL)P009-APC-500