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CF® Dye TCO

CF® dye with TCO (trans-cyclooctene) groups react with tetrazine-labeled molecules via copper-free click chemistry.

Product Attributes

Chemical reactivity (reacts with)

Tetrazine

Functional group

TCO (trans-cyclooctene)

Storage Conditions

Store at -10 to -35 °C, Protect from light

Conjugation
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Product Description

CF® dye with TCO group reacts with tetrazine containing molecules via copper-free click chemistry. Copper-free bioorthogonal reactions are useful for labeling live cells, or when there are concerns about native protein function loss with copper in cell extracts. Membrane-permeant CF® dye methyltetrazine options are also available for intracellular labeling reactions.

Available CF® dye TCO derivatives: CF®488A, CF®532, CF®555, CF®568, CF®594, CF®640R, CF®647, CF®680, and CF®680R including membrane-permeant CF®500, and CF®650 TCO.

  • Fluorescent labeling of tetrazine groups on target molecules via copper-free bioorthonal chemistry.
  • Membrane-impermeant and membrane-permeant forms for  surface or intracellular labeling of live cells or cell extracts.
  • Bright, photostable and water-soluble CF® dyes are excellent options for fluorescent labeling.

         

We also offer CF® dye tetrazine and CF® dye methyltetrazine.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

CF Dye TCO: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
CF®488A TCOCF®488A490/515 nm1 mg96040Purchase 96040
CF®500 TCOCF®500500/510 nm1 mg96041Purchase 96041
CF®532 TCOCF®532527/558 nm1 mg96042Purchase 96042
CF®555 TCOCF®555555/565 nm1 mg96043Purchase 96043
CF®568 TCOCF®568562/583 nm1 mg96044Purchase 96044
CF®594 TCOCF®594593/614 nm1 mg96045Purchase 96045
CF®640R TCOCF®640R642/662 nm1 mg96046Purchase 96046
CF®647 TCOCF®647650/665 nm1 mg96047Purchase 96047
CF®650 TCOCF®650650/670 nm1 mg96048Purchase 96048
CF®680 TCOCF®680681/698 nm1 mg96051Purchase 96051
CF®680R TCOCF®680R680/701 nm1 mg96049Purchase 96049