CF® dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) containing molecules via copper free click chemistry. This copper free bioorthogonal reaction is useful for labeling live cells, or when there are concerns about native protein function loss with copper in cell extracts.
CF® dyes are exceptionally bright and photostable, making them the perfect dyes for fluorescence detection. The following CF® dyes are available with methyltetrazine: CF®488A, CF®500, CF®532, CF®555, CF®568, CF®594, CF®640R, CF®647, CF®650, CF®680, and CF®680R.
CF®500 methyltetrazine and CF®650 methyltetrazine are designed to use for intracellular copper free reaction with cyclooctene. CF®488A, CF®532, CF®555, CF®568, CF®594, CF®640R, CF®647, CF®680, and CF®680R are cell membrane-impermeant.
CF® dye methyltetrazines have improved chemical stability compared to CF® dye tetrazines. We also offer CF® dye TCO.
CF Dye Methyltetrazine: Product & SKU Summary