Support & Resources

+1

CF® Dye Methyltetrazine

CF® Dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

Product Attributes

Chemical reactivity (reacts with)

Cyclooctene (TCO, methylcyclopropene) groups

Functional group

Tetrazine/Methyltetrazine

Storage Conditions

Store at -10 to -35 °C, Protect from light

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Size
Catalog #
price
Qty
1 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Product Description

CF® Dye with a methyltetrazine group react with cyclooctene (TCO, methylcyclopropene) containing molecules via copper-free click chemistry. This copper-free bioorthogonal reaction is useful for labeling live cells, or when there are concerns about native protein function loss with copper in cell extracts. Membrane-permeant CF® Dye methyltetrazine options are also available for intracellular labeling reactions.

Available CF® Dye methyltetrazine derivatives: CF®488A, CF®532, CF®555, CF®568, CF®594, CF®640R, CF®647, CF®680, and CF®680R including membrane-permeant CF®500, and CF®650 methyltetrazine.

  • Fluorescent labeling of cyclooctene (TCO, methylcyclopropene) groups on target molecules via copper-free bioorthonal chemistry.
  • Membrane-impermeant and membrane-permeant forms for  surface or intracellular labeling of live cells or cell extracts.
  • Bright, photostable and water-soluble CF® Dyes are excellent options for fluorescent labeling.

         

CF® Dye methyltetrazines have improved chemical stability compared to CF® Dye tetrazines. We also offer CF® Dye TCO.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

CF Dye Methyltetrazine: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
CF®488A MethyltetrazineCF®488A490/515 nm1 mg96028Purchase 96028
CF®500 MethyltetrazineCF®500500/510 nm1 mg96029Purchase 96029
CF®532 MethyltetrazineCF®532527/558 nm1 mg96030Purchase 96030
CF®555 MethyltetrazineCF®555555/565 nm1 mg96031Purchase 96031
CF®568 MethyltetrazineCF®568562/583 nm1 mg96032Purchase 96032
CF®594 MethyltetrazineCF®594593/614 nm1 mg96033Purchase 96033
CF®640R MethyltetrazineCF®640R642/662 nm1 mg96034Purchase 96034
CF®647 MethyltetrazineCF®647650/665 nm1 mg96035Purchase 96035
CF®650 MethyltetrazineCF®650650/670 nm1 mg96036Purchase 96036
CF®680 MethyltetrazineCF®680681/698 nm1 mg96039Purchase 96039
CF®680R MethyltetrazineCF®680R680/701 nm1 mg96037Purchase 96037
 

You may also like…

    CF® Dye Tetrazine

    CF® Dyes with a tetrazine group react with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

    CF® Dye Alkyne

    CF® Dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.

    CF® Dye Azide

    CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

    CF® Dye BCN

    CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.

    CF® Dye Picolyl Azide

    CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.

    CF® Dye SE/TFP Ester

    CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.