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CF® Dye Azide

CF® dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

Product Attributes

Chemical reactivity (reacts with)

Alkynes, BCN

Functional group

Azide

Storage Conditions

Store at -10 to -35 °C, Protect from light

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

CF® dye azide can react with terminal alkynes to form 1,2,3-triazole via copper-catalyzed cycloaddition. They can also undergo Staudinger ligation with a phosphine containing compounds. The azide derivatives also react with cyclooctynes and BCN groups via copper-free bioorthogonal reaction.

  • Fluorescent detection or labeling of alkyne, BCN or cyclooctyne groups on target molecules.
  • Bright, photostable and water-soluble CF® dyes are excellent options for fluorescent labeling.

Superior CF® Dyes
CF® dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability and water solubility compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.

Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

We also offer CF® dye Picolyl Azide derivatives for azide-alkyne cycloaddition reactions at lower copper concentrations, suitable for labeling live cells or other copper-sensitive systems.

CF® Dye Azide

ProductEx/EmSizeCatalog No.
CF®405M Azide408/452 nm0.5 mg92092
CF®488A Azide490/515 nm0.5 mg92080
CF®532 Azide527/558 nm0.5 mg92180
CF®543 Azide541/560 nm0.5 mg92181
CF®555 Azide555/565 nm0.5 mg92081
CF®568 Azide562/583 nm0.5 mg92082
CF®594 Azide593/614 nm0.5 mg92083
CF®640R Azide642/662 nm0.5 mg92085
CF®647 Azide650/665 nm0.5 mg92084
CF®660C Azide667/685 nm0.5 mg92094
CF®660R Azide663/682 nm0.5 mg92182
CF®680 Azide681/698 nm0.5 mg92119
CF®680R Azide680/701 nm0.5 mg96000

References

Nat Protocols (2017) 12(5), 1011-1028. doi: 10.1038/nprot.2017.020 (CF®405M azide, CF®568 azide, SIM)

Download a list of CF® dye references.

 

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    CF® Dye BCN

    CF® dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® dye BCN conjugates for intracellular labeling reactions are also available.

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    CF® Dye Succinimidyl Ester

    CF® dye SE or NHS esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

    CF® Dye Picolyl Azide

    CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.

    Biotin BCN

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.

    CF® Dye Alkyne

    CF® dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® dye alkynes can also be used as building blocks to form fluorescent polymers.