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CF® Dye Alkyne

CF® dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® dye alkynes can also be used as building blocks to form fluorescent polymers.

Product Attributes

Chemical reactivity (reacts with)

Azides/Picolyl azides

Functional group


Storage Conditions

Store at -10 to -35 °C, Protect from light

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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0.5 mg
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Product Description

CF® dye alkynes can react with azide groups to form 1,2,3-triazole via 1,3-dipolar Huisgen copper-catalyzed cycloaddition. They can also be used as monomeric building blocks to copolymerize with other monomers to form fluorescent polymers.

  • Fluorescent detection or labeling azide groups on target molecules.
  • Can be used as fluorescent monomers to form dye-labeled polymers.
  • Bright, photostable and water-soluble CF® dyes are excellent options for fluorescent labeling.

Superior CF® Dyes
CF® dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability and water solubility compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.

Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

CF Dye Alkyne: Product & SKU Summary

CF®405M AlkyneCF®405M408/452 nm0.5 mg92093Purchase 92093
CF®488A AlkyneCF®488A490/515 nm0.5 mg92086Purchase 92086
CF®555 AlkyneCF®555555/565 nm0.5 mg92087Purchase 92087
CF®568 AlkyneCF®568562/583 nm0.5 mg92088Purchase 92088
CF®594 AlkyneCF®594593/614 nm0.5 mg92089Purchase 92089
CF®640R AlkyneCF®640R642/662 nm0.5 mg92091Purchase 92091
CF®647 Alkyne
650/665 nm
0.5 mg
92090Purchase 92090
CF®660C AlkyneCF®660C667/685 nm0.5 mg92095Purchase 92095
CF®660R AlkyneCF®660R663/682 nm0.5 mg96004Purchase 96004
CF®680 AlkyneCF®680681/698 nm0.5 mg96005Purchase 96005
CF®680R AlkyneCF®680R680/701 nm0.5 mg96006Purchase 96006


1. Dev Cell. (2019) 50(1):57-72.e6. DOI: 10.1016/j.devcel.2019.04.035
2. ACS Nano (2016) 10:8861−8870. DOI: 10.1021/acsnano.6b04748

Download a list of CF® dye references.


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    CF® Dye Azide

    CF® dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

    CF® Dye Picolyl Azide

    CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.

    CF® Dye BCN

    CF® dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® dye BCN conjugates for intracellular labeling reactions are also available.

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    Biotin BCN

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.

    CF® Dye Succinimidyl Ester

    CF® dye SE or NHS esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.