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CF® Dye Alkyne

CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers.

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Product Description

CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers.

CF Dye Alkyne: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
CF®405M AlkyneCF®405M408/452 nm0.5 mg92093Purchase 92093
CF®488A AlkyneCF®488A490/515 nm0.5 mg92086Purchase 92086
CF®555 AlkyneCF®555555/565 nm0.5 mg92087Purchase 92087
CF®568 AlkyneCF®568562/583 nm0.5 mg92088Purchase 92088
CF®594 AlkyneCF®594593/614 nm0.5 mg92089Purchase 92089
CF®640R AlkyneCF®640R642/662 nm0.5 mg92091Purchase 92091
CF®647 Alkyne
CF®647
650/665 nm
0.5 mg
92090Purchase 92090
CF®660C AlkyneCF®660C667/685 nm0.5 mg92095Purchase 92095
CF®660R AlkyneCF®660R663/682 nm0.5 mg96004Purchase 96004
CF®680 AlkyneCF®680681/698 nm0.5 mg96005Purchase 96005
CF®680R AlkyneCF®680R680/701 nm0.5 mg96006Purchase 96006

References

Download a list of CF® dye references.

 

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    CF® Dye Azide

    CF® dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

    CF® Dye Picolyl Azide

    CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.

    CF® Dye BCN

    CF® dye with BCN reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper.

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    Biotin BCN

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells or having concerns about native protein function loss with copper in cell extracts.

    CF® Dye Succinimidyl Ester

    CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues.