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CF® Dye Picolyl Azide

CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.

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Product Description

CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.

Picolyl azide is available with the following CF® dyes: CF®488A, CF®568, CF®594, CF®640R, CF®647, CF®660C, CF®680, and CF®680R.

CF Dye Picolyl Azide: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
CF®488A Picolyl AzideCF®488A490/515 nm0.5 mg92187Purchase 92187
CF®568 Picolyl AzideCF®568562/583 nm0.5 mg92188Purchase 92188
CF®594 Picolyl AzideCF®594593/614 nm0.5 mg92189Purchase 92189
CF®640R Picolyl AzideCF®640R642/662 nm0.5 mg92190Purchase 92190
CF®647 Picolyl AzideCF®647650/665 nm0.5 mg92191Purchase 92191
CF®660C Picolyl AzideCF®660C667/685 nm0.5 mg96001Purchase 96001
CF®660R Picolyl AzideCF®660R663/682 nm0.5 mg96002Purchase 96002
CF®680 Picolyl AzideCF®680681/698 nm0.5 mg96003Purchase 96003
CF®680R Picolyl AzideCF®680R680/701 nm0.5 mg96007Purchase 96007

References

Download a list of CF® dye references.

 

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    CF® dye with BCN reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper.

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    CF® Dye Azide

    CF® dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

    Biotin BCN

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells or having concerns about native protein function loss with copper in cell extracts.

    CF® Dye Alkyne

    CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers.