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CF® Dye Tetrazine

CF® Dyes with a tetrazine group react with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

Product Attributes

Chemical reactivity (reacts with)

Cyclooctene (TCO, methylcyclopropene) groups, TCO

Functional group

Tetrazine/Methyltetrazine

Storage Conditions

Store at -10 to -35 °C, Protect from light

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Product Description

Tetrazine CF® Dyes react with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry. These copper-free bioorthogonal reactions are useful for labeling fixed cells or when there are concerns about native protein function loss with copper in cell extracts.

  • Fluorescent labeling of cyclooctene (TCO, methylcyclopropene) groups on target molecules via copper-free bioorthonal chemistry.
  • Faster reaction kinetics than methyltetrazine derivatives.
  • Bright, photostable, and water-soluble CF® Dyes are excellent options for fluorescent labeling.

We also offer CF® Dye Methyltetrazines and CF® Dye TCO.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

CF® Dye Tetrazine

DyeEx/EmSizeCatalog No.Dye Features
CF®488A490/516 nm1 mg96054CF®488A Features
CF®568562/584 nm1 mg96055CF®568 Features
CF®647650/668 nm1 mg96056CF®647 Features
CF®660C667/685 nm1 mg96096CF®660C Features
CF®680681/698 nm1 mg96097CF®680 Features
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.
 

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    CF® Dye Azide

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    CF® Dye BCN

    CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.

    CF® Dye Methyltetrazine

    CF® Dye with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

    CF® Dye Picolyl Azide

    CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.

    CF® Dye TCO

    CF® Dye with TCO (trans-cyclooctene) groups react with tetrazine-labeled molecules via copper-free click chemistry.