DMNP-EDTA, Tetrapotassium Salt (Caged Calcium)
DMNP-EDTA (also known as DM-Nitrophen™) is a caged calcium chelator. Upon photolysis, the Kd for Ca2+ increases from 5 nM to 3 mM, resulting in a pulse of free Ca2+.
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Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
Calcium Calibration Buffer Kit
This kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca2 indicators (1,2). This product contains 50 mL each of components A and B.
Probenecid, Sodium Salt, Water Soluble
Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to low signal or high background in assays due to poor cellular retention of dyes.
Fluo-4 AM Ester
Membrane-permeant form of Fluo-4 calcium indicator. Fluo-4 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-4 by endogenous esterases once inside cells.
Fluo-3, AM Ester
Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading.