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dUTP CF® Dye Conjugates

Labeled with our superior CF® dyes, dUTP conjugates can be used for TUNEL assay or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.

Product Attributes

Applications

DNA Probe, Microscopy, Flow cytometry, In situ hybridization, Gel imaging

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labeled DNA probes for in situ hybridization and nucleic acid blotting applications.

  • Synthesize exceptionally bright and photostable fluorescent DNA probes
  • Detect by fluorescence microscopy, flow cytometry, or in gel
  • Compatible with fixed cells or tissue sections
  • Choice of 8 CF® dye colors from blue to near infrared
  • Biotium’s CF® dyes have superior brightness, photostability, and are water-soluble

Supplied in lyophilized form. For PCR applications, Taq polymerase should be used with dUTP conjugates, because dUTP inhibits archaeal polymerases such as Pfu and Vent®. Also see our CF® Dye dCTP Conjugates and TUNEL assay kits, available with a wide selection of bright and photostable CF® dyes.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

dUTP CF® Dye Conjugates

ProductDyeEx/EmSizeSKUPurchase
CF®405S dUTP1CF®405S404/431 nm5 nmol40004-TPurchase 40004-T
25 nmol40004Purchase 40004
CF®405M dUTP CF®405M408/452 nm5 nmol40100-TPurchase 40100-T
25 nmol40100Purchase 40100
CF®488A dUTP CF®488A490/515 nm5 nmol40008-TPurchase 40008-T
25 nmol40008Purchase 40008
CF®543 dUTP CF®543541/560 nm5 nmol40002-TPurchase 40002-T
25 nmol40002Purchase 40002
CF®568 dUTP CF®568562/583 nm5 nmol40005-TPurchase 40005-T
25 nmol40005Purchase 40005
CF®594 dUTP CF®594593/614 nm5 nmol40006-TPurchase 40006-T
25 nmol40006Purchase 40006
CF®640R dUTPCF®640R642/662 nm5 nmol40007-TPurchase 40007-T
25 nmol40007Purchase 40007
CF®680R dUTP2CF®680R680/701 nm5 nmol40003-TPurchase 40003-T
25 nmol40003Purchase 40003
1. CF®405S-dUTP may not be suitable for TUNEL staining in tissues due to blue autofluorescence in tissues and lower incorporation efficiency in tissue sections compared to other CF®dye dUTP conjugates. 2. CF®680R-dUTP has been tested in TUNEL staining of cells, but may not be efficiently incorporated in tissue sections.

 

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific. Vent is a registered trademark of New England Biolabs, Inc.

References

Download a list of CF® dye references.

  1. Nat Commun, 10, (2019), 967; DOI: 10/gfv3m9
  2. Molecular Cell, 75, (2019), 267; DOI: 10/ggvb22
  3. bioRxiv, (2019), 536136; DOI: 10/ggvb2w
  4. Nat Commun, 7, (2016), 10966; DOI: 10/f8dxfd
  5. Nucleus, 7, (2016), 325; DOI: 10/ggvb24
  6. Cell Death Differ, 19, (2012), 245; DOI: 10/btnptc
 

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