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RedDot™1 Far-Red Nuclear Stain, 200X in Water

A far-red live cell nuclear stain similar to Draq5™.

Product Attributes

Cellular localization

Nucleus

Assay type/options

DNA content/cell cycle (flow cytometry), Short term staining (<24h), No-wash staining

Cell permeability

Membrane permeant

Colors

Far-red

Excitation/Emission

662 (broad)/694 nm (with DNA)

Concentration

200X in water

Storage Conditions

Store at -10 to -35 °C, Protect from light

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Product Description

RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.

  • Ideal for specifically staining the nuclei of live cells for short term imaging
  • Highly thermostable and photostable, for convenient handling and demanding imaging applications
  • Can be used for DNA content analysis by flow cytometry like Vybrant™ DyeCycle™ Ruby
  • Can be used for normalization of In Cell Western® in fixed cells, similar to Draq5™/Sapphire®700
  • λExEm = 662/694 nm (with DNA), for detection in the Cy®5 channel

RedDot™1 can be excited at a wide range of wavelengths between 488 nm and 647 nm, including the 488 nm laser for flow cytometry, and can be used for DNA content analysis by flow cytometry. While DAPI and Hoechst dyes show a strong preference for A-T rich regions, RedDot™1 is relatively insensitive to sequence base composition.

Choose the Right Dye for Your Application

Like Draq5®, RedDot™1 is toxic to cells within a few hours, and is recommended for short term staining experiments. For a low-toxicity far-red nuclear stain for live cells, see our NucSpot™ 650 dye.

RedDot™1 can be used to stain live bacteria (gram-positive and gram-negative). Staining in live yeast is not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.

RedDot™1 cannot be used for nuclear-specific staining in fixed cells. Please see RedDot™2 (cat# 40061), a spectrally similar dye designed for specific nuclear staining of fixed and permeabilized cells or selective nuclear staining of dead cells.

Please note: far-red dyes like RedDot™1 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.



RedDot™1 and RedDot™2 Far-Red Nuclear Stains

ProductCatalog No.Cell PermeabilityLive/FixedColor (Ex/Em)Applications
RedDot™1 Far-Red Nuclear Stain, 200X in Water40060PermeantLive cells only(Far-red)
662/694 nm
• Live cell nuclear stain comparable to Draq5™
• For microscopy, cell cycle analysis by flow cytometry
• Can be used for In-Cell Western normalization
RedDot™2 Far-Red Nuclear Stain, 200X in DMSO40061ImpermeantFixed/permeabilized cells or tissues(Far-red)
665/695 nm
• Fixed cell nuclear stain comparable to Draq7™
• Nuclear-specific with minimal cytoplasmic staining
• Selectively stains dead cells in unfixed cultures
Cy dye is a registered trademark of GE Healthcare. Draq5 is a trademark of BioStatus Ltd. In Cell Western and Sapphire are registered trademarks of LI-COR Inc. Vybrant and DyeCycle are trademarks of Thermo Fisher Scientific.

References

Download a list of RedDot™1 References.

 

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