RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells for short term imaging. RedDot™1 can be used to stain adherent or suspension cells. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. While DAPI and Hoechst dyes show a strong preference for A-T rich regions, RedDot™1 is relatively insensitive to sequence base composition. RedDot™1 has excitation/emission maxima at 662/694 nm, for detection in the Cy®5 channel. It also can be excited at a wide range of wavelengths between 488 nm and 647 nm, including the 488 nm laser for flow cytometry, and can be used for DNA content analysis by flow cytometry.
Like Draq5®, RedDot™1 is toxic to cells within a few hours, and is recommended for short term staining experiments. For a low-toxicity far-red nuclear stain for live cells, see our NucSpot™ 650 dye.
RedDot™1 also can be used for normalization of In Cell Western® in fixed cells, similar to Draq5™/Sapphire®700. Note that the dye cannot be used for nuclear-specific staining in fixed cells. Please see RedDot™2 (cat# 40061), a spectrally similar dye designed for specific nuclear staining of fixed and permeabilized cells or selective nuclear staining of dead cells.
RedDot™1 can be used to stain live bacteria (gram-positive and gram-negative). Staining in live yeast is not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Please note: far-red dyes like RedDot™1 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.
Cy dye is a registered trademark of GE Healthcare. Draq5 is a trademark of BioStatus Ltd. In Cell Western and Sapphire are registered trademarks of LI-COR Inc.
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