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DMAO, 2 mM in DMSO

DMAO, 2 mM in DMSO is a green-fluorescent nucleic acid dye for staining live bacteria.

Product Attributes

Cellular localization

Nucleus & cytoplasm

For live or fixed cells

For live/intact cells

Cell permeability

Membrane permeant

Colors

Green

Excitation/Emission

496/528 nm (with DNA)

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1 mL
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Protocols
SDS

Product Description

DMAO is a membrane-permeable DNA dye, excellent for staining live bacteria. It is able to stain both gram positive and gram negative strains, and stained cells can be detected by fluorescence microscopy or flow cytometry.

We also offer DMAO paired together with a dead cell stain (Ethidium Homodimer III, or EthD-III) in our Viability/Cytotoxicity Assay Kit for Bacteria Live and Dead Cells (30027).

DMAO staining in live yeast is not nuclear. In live mammalian cells, DMAO shows both nuclear and cytoplasmic staining. We recommend NucSpot® Live or RedDot™1 dyes for nuclear staining of live mammalian cells. See our Cellular Stains Table for more information on how our dyes stain various organisms.

DMAO preferentially binds to dsDNA with green fluorescence (Ex/Em, DNA-bound = 496/528 nm). Unlike the SYBR dyes, DMAO is extremely stable and can be routinely handled and stored at room temperature without degradation.

  • Green DNA stain for live bacteria
  • Stable for room temperature storage
  • λExEm (with DNA) = 496/528 nm
  • Supplied at 2 mM in DMSO
  • Supplied at 1 mL

We also offer BactoView™ Live bacteria stains in two colors (green & red). BactoView™ Live dyes give the most consistent, bright staining of bacteria of different strains, with minimal difference between live and dead cells.

References

n/a

 

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