Fluorescamine is nonfluorescent until it reacts with primary amines. The reaction is completed in miniseconds and can be used to detect amine-containing compounds including peptides and proteins.
You may also like…
CF® Dye Succinimidyl Ester
CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues.
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.
Cyanine Dye Succinimidyl Ester
Biotium offers high quality amine-reactive succinimidyl ester (SE) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555 (Cy®3), Cyanine 647 (Cy®5), Cyanine 680 (Cy®5.5), and Cyanine 750 (Cy®7) as high quality succinimidyl esters.
CF® Dye & Biotin SE Protein Labeling Kits
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE) or biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction.
CFQ520 succinimidyl ester
The amine-reactive quencher, CFQ520 succinimidyl ester, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.