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Live-or-Dye NucFix™ Red Staining Kit

A chemically-reactive, dead cell nuclear stain for mammalian cells, yeast, or gram-negative bacteria.

Product Attributes

Apoptosis/viability marker

Dead cell stain

For live or fixed cells

Covalent & fixable stains, For live/intact cells

Assay type/options

Endpoint assay

Detection method/readout

Fluorescence microscopy, Flow cytometry

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining

Colors

Red

Excitation/Emission

520/593 nm (with DNA)

Size
Catalog #
price
Qty
50 assays
200 assays
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Product Description

Features:

  • Nuclear-specific dead cell stain for flow cytometry or microscopy
  • Covalent labeling withstands fixation and permeabilization
  • Red fluorescence (λExEm = 520/593 nm)
  • Selective dead-cell staining of mammalian cells, yeast*, or gram-negative bacteria

Biotium’s line of Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis. In microscopy, live/dead stains allow unambiguous visual discrimination of dead cells.

Live-or-Dye NucFix™ Red is a chemically-reactive, cell membrane impermeable dye that specifically stains the nuclei of dead cells. The dye is able to enter into dead cells that have compromised membrane integrity and covalently label the cell nucleus, allowing for clear differentiation of live and dead cells by either microscopy or flow cytometry. Unlike other commonly used nuclear stains such as Propidium Iodide or DRAQ7™, NucFix™ labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye NucFix™ Red staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.

Live-or-Dye NucFix™ Red can also be used as a fixable dead cell stain in gram-negative bacteria. In gram-positive strains there may be live cell staining. *Note: Live-or-Dye NucFix™ Red is dead cell specific but not nuclear in yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Kit contents:


50 assay trial size:

  • 1 vial lyophilized dye (50 labeling reactions)
  • Anhydrous DMSO for dye reconstitution

200 assay size:

  • 4 vials lyophilized dye (50 labeling reactions per vial)
  • Anhydrous DMSO for dye reconstitution

Live-or-Dye™ Fixable Viability Staining Kits

Catalog No.

Viability Dye

Laser line

Emission filter

Abs/Em Max

Applications

32002, 32002-TLive-or-Dye™ 350/448355 nmDAPI or Violet347/448 nmFlow Cytometry
32003, 32003-TLive-or-Dye™ 405/452405 nmPacific Blue®408/452 nmFlow Cytometry
32009, 32009-TLive-or-Dye™ 405/545405 nmAmCyan395/545 nmFlow Cytometry,
Microscopy
32004, 32004-TLive-or-Dye™ 488/515488 nmFITC490/515 nmFlow Cytometry,
Microscopy
32012, 32012-TLive-or-Dye™ 510/550488 nmSpectral scan516/549 nmSpectral Cytometry
32005, 32005-TLive-or-Dye™ 568/583488 or 561 nmPE562/583 nmFlow Cytometry,
Microscopy
32006, 32006-TLive-or-Dye™ 594/614488 or 561 nmPE-Texas Red®593/614 nmFlow Cytometry,
Microscopy
32007, 32007-TLive-or-Dye™ 640/662633 or 640 nmAPC642/662 nmFlow Cytometry
32013, 32013-TLive-or-Dye™ 665/685633 or 640 nmSpectral scan667/685 nmSpectral Cytometry
32008, 32008-TLive-or-Dye™ 750/777633 or 640 nmAPC-Cy®7755/777 nmFlow Cytometry,
Microscopy
32010, 32010-TLive-or-Dye NucFix™ Red488 or 532 nmPE-Texas Red®520/593 nmFlow Cytometry,
Microscopy

 

Draq7 is a trademark of Biostatus Ltd.

References

Cell Transplantation, 2019. https://doi.org/10.1177/0963689719866685
Access Microbiology, 2019. https://doi.org/10.1099/acmi.ac2019.po0242

 

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