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Mix-n-Stain™ Enzyme Antibody Labeling Kits

Mix-n-Stain™ enzyme antibody labeling kits are a revolutionary antibody labeling technology that allows you to label up to 100 ug of your antibody with HRP, alkaline phosphatase, or glucose oxidase with minimal hands-on time and without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers.

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10-20 ug labeling
25-50 ug labeling
50-100 ug labeling
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Product Description

Mix-n-Stain™ enzyme antibody labeling kits are a revolutionary antibody labeling technology that allows you to label up to 100 ug of your antibody with an enzyme with minimal hands-on time and no purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. For more information, please download the product information sheets for the kits.

This kit is available with the following enzymes: HRP (horseradish peroxidase), glucose oxidase, and alkaline phosphatase.

• Horseradish peroxidase conjugates can be used for Western blot, ELISA, immunohistochemistry and other standard immunoassay applications.  The enzyme label can be visualized with chromogenic substrates such as DAB, ABTS, TMB and TMBUS in the presence of hydrogen peroxide. Label 10-20 ug, 25-50 ug, or 50-100 ug of antibody in about 3 hours.

• Glucose oxidase is an enzyme which catalyses the oxidation of glucose with the release of hydrogen peroxide. Label 25-50 ug, or 50-100 ug of antibody in 2 hours.

• Alkaline phosphatase conjugates can be used for ELISA, immunohistochemistry and other standard immunoassay applications.  The enzyme label can be visualized with chromogenic substrates such as BCIP and PNPP as well as fluorogenic substrate such as MUP. Label 25-50 ug, or 50-100 ug of antibody in 2 hours.

Mix-n-Stain™ Enzyme Antibody Labeling Kits

Product descriptionCatalog numberReaction size Product protocolLabeling time
Mix-n-Stain™ HRP Antibody Labeling Kit9230010-20 ug antibody
PI-Mix-n-Stain™ HRP
~3.5 hours (few minutes hands-on time)
9230125-50 ug antibody
9230250-100 ug antibody
Mix-n-Stain™ Alkaline Phosphatase Antibody Labeling Kit9231425-50 ug antibody
PI-Mix-n-Stain™ AP
~2 hours (few minutes hands-on time)
9231550-100 ug antibody
Mix-n-Stain™ Glucose Oxidase Antibody Labeling Kit9231225-50 ug antibody
PI-Mix-n-Stain™ GOx
9231350-100 ug antibody

References

Download a list of Mix-n-Stain™ references.

 

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