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Mix-n-Stain™ Biotin Antibody Labeling Kit

Label your antibody with biotin in just 30 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Catalog #
3x(5-20 ug) labelings
5-20 ug labeling
20-50 ug labeling
50-100 ug labeling
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Product Description

Mix-n-Stain™ Biotin Antibody Labeling Kits allow you to biotinylate ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification.


  • Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification, 100% recovery
  • Compatible with BSA, gelatin, ascites

Kit components

  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive Biotin
  • Mix-n-Stain™ Storage Buffer
Overview of Mix-n-Stain Labeling Protocol
Overview of the Mix-n-Stain™ Antibody Labeling Kit workflow. Click to enlarge.

Unrivaled Convenience

Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. The presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.

Choose the Right Labeling Kit for Your Antibody

Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.

We offer Mix-n-Stain™ Kits for labeling antibodies, nanobodies, and small ligands with a wide selection of fluorescent dyes, enzymes, and other labels. See our full selection of Mix-n-Stain™ Antibody Labeling Kits below.

Mix-n-Stain™ Product Lines

Mix-n-Stain™ CF® Dye
Antibody Labeling Kits
CF® Dyes≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG
~ 30 min.• Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits
Fluorescein (FITC)
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits
Cyanine 555 (Cy®3)
Cyanine 647 (Cy®5)
Mix-n-Stain™ Biotin
Antibody Labeling Kits
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits
Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits
Dinitrophenol (DNP)
Fluorescent Protein
& Tandem Dye
Antibody Labeling Kits
Tandem Dyes
25-50 ug IgG
50-100 ug IgG
~ 4 hours• Fast, simple labeling

• Minimal hands-on time

• No purification
Mix-n-Stain™ Enzyme
Antibody Labeling Kits
10-20 ug IgG
25-50 ug IgG
50-100 ug IgG

AP or GOx:
25-50 ug IgG
50-100 ug IgG
~ 2-3 hours
Mix-n-Stain™ Maxi
Antibody Labeling Kits
CF® Dyes
Cy® Dyes
1 mg IgG~ 30 min.
Nanobody Labeling Kits
CF® Dyes5-20 ug Nanobody®
20-50 ug Nanobody®
~ 30 min.• Optimized for Nanobodies®

• Tolerates BSA & other additives
Small Ligand Labeling Kits
CF® Dyes0.1 umol
small ligand
~ 30 min.• Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
CF® Dye & Biotin SE
Protein Labeling Kits
CF® Dyes
3 x 1 mg
~ 2 hours• Dyes, buffers, & spin vials for labeling + purification
Note: Kits are not recommended for labeling IgM antibodies. Cy dye is a registered trademark of GE Healthcare.


Download a list of Mix-n-Stain™ references.


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