Polyclonal anti Beta Catenin (p120) (Rabbit PAb)

BNUB0376-0
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Product Description

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Dabbs DJ et. al. Am J Surg Path. 2007;31:427-437. | Sarrio D et. al. Oncogene. 2004;23:3272-3283. | Mastracci TL et. al. Mod Path. 2005;18:741-751

Additional Information

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Rabbit

Reactivity (target)

Beta Catenin (p120) (Rabbit PAb)

Clonality

Polyclonal

Isotype

IgG

Clone

Rabbit PAb

Molecular weight

92 kDa

Synonyms

Cadherin associated protein), beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Entrez gene ID

1499

SwissProt

P35222

Unigene

476018

Immunogen

A synthetic peptide from the middle of beta-Catenin (p120) protein

Species reactivity

Human, Mouse

Cellular localization

Cell surface and cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 1-2 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL

Positive control

HeLa or MCF-7 cells. Breast carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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