Monoclonal anti CD66, pan (CEA) (C66/195 + C66/261 + C66/1009 + C66/1030)

Price:

from

$120.00

Catalog #: BNUB1139

Selection:

Multiple sizes

Category: Primary Antibodies.

>>> Order 5 or more and receive 10% off.

Description
References
More Info
Downloads
Shipping/shelf life

Product Description

This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Muraro R, et. al. Cancer Research, 1985, 45:5769-80. | Siler K, et. al. Biotechnology Therapeutics, 1993, 4(3-4):163-81. | Robbins PF, et. al. International Journal of Cancer, 1993, 53(6):892-7. | Shi ZR, et. al. Journal of Histochemistry and Cytochemistry, 1994, 42(9):1215-9

Additional Information

Conjugation	Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP
Size	50 uL, 100 uL, 250 uL, 500 uL
Supplied As	Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA
Antibody type	Primary
Host species	Mouse
Reactivity (target)	CD66, pan (CEA) (C66/195 + C66/261 + C66/1009 + C66/1030)
Clonality	Monoclonal
Isotype	IgG's
Clone	C66/195 + C66/261 + C66/1009 + C66/1030
Molecular weight	80-200 kDa
Synonyms	Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)
Entrez gene ID	1048 & 634
SwissProt	P06731
Unigene	709196
Immunogen	Recombinant full-length human CEA protein (C66/195; C66/261; C66/1009 & C66/1030)
Species reactivity	Human
Applications	Flow cytometry, Immunofluorescence, Immunohistology (formalin)
Cellular localization	Cytoplasmic and luminal surface
Application notes	Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 min at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Positive control	MCF7 or 293T cells. Colon carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life