Monoclonal anti CD86 (C86/1146)

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$120.00

Catalog #: BNUB1146

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Category: Primary Antibodies.

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Product Description

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin’s disease.

n/a

Additional Information

Applications	Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western
Conjugation	Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP
Size	50 uL, 100 uL, 250 uL, 500 uL
Supplied As	Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA
Antibody type	Primary
Host species	Mouse
Reactivity (target)	CD86 (C86/1146)
Clonality	Monoclonal
Isotype	Mouse/IgG s
Clone	C86/1146
Molecular weight	70 kDa
Entrez gene ID	942
SwissProt	P42081
Immunogen	Recombinant human full-length CD86 protein
Unigene	171182
Species reactivity	Human, Mouse, Rat
Cellular localization	Cell surface
Application notes	Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 2-4 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL
Positive control	293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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