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CD98 Monoclonal Mouse Antibody (UM7F8)

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Product Attributes

Antibody number

#0425

Antibody reactivity (target)

CD98

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

UM7F8

Isotype

IgG1

Molecular weight

Heterodimer (125 kDa) made of a disulfide-linked glycosylated heavy chain of approximately 80-90 kDa and a non-glycosylated light chain

Synonyms

4F2, 4F2HC, 4T2HC, CD98HC; MDU1; NACAE; Slc3a2; Solute Carrier Family 3 (activators of dibasic and neutral amino acid transport) Member 2, E16, LAT1, MPE16, hLAT1

Human gene symbol

SLC3A2

Entrez gene ID

6520

SwissProt

P08195

Unigene

502769

Immunogen

Molt 13 T cell line

Antibody target cellular localization

Plasma membrane

Expected antibody applications

Flow, surface (published for clone), Functional studies (published for clone), IF (published for clone), IP (published for clone)

Species reactivity

Human

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 0.5-1.0 ug/mL, Comitogenic with soluble anti-CD2 and immobilized anti-CD3 mAbs, Optimal dilution for a specific application should be determined by user

Positive control

HL-60, Jurkat, MG63, HUT-78, K562, YT, U937, Hep-G2 cells, Tonsil

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Transporters

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-StainTM Antibody Labeling Kits for labeling antibodies with all CF® Dyes. We apologize for the inconvenience.
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Product Description

CD98 exits as a heterodimer containing a disulphide-linked glycosylated heavy chain and a non-glycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through disulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0425, Anti-CD98 (UM7F8) 

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Note: References for this clone sold by other suppliers may be listed for expected applications.

  1. Cell Immunol (1994) 154(1):253-63. (IP, Flow, functional studies)
  2. J Cell Biol (2001) 276(42): 39282-39289. (IF)
  3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 280-283.
  4. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 531-534.
 

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