Monoclonal anti Cytokeratin 5/8 (C 50)

BNUB0948-0
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Catalog #: BNUB0948
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Product Description

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Cytokeratin 5/8 (C-50)

Clonality

Monoclonal

Isotype

IgM, kappa

Clone

C-50

Molecular weight

58 kDa (CK5); 52.5 kDa (CK8)

Synonyms
Entrez gene ID

3852 (CK5); 3856 (CK8)

SwissProt

P13647 (CK5); P05787 (CK8)

Unigene

433845 (CK5); 533782 & 708445 (CK8)

Immunogen

Cytoskeletal preparation from HeLa cells

Species reactivity

Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Cytoplasmic

Application notes

Does not react with chicken , or Xenopus, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/mL PBS, 15 min at RT, Immunofluorescence 0.5-1.0 ug/mL, Immunohistology formalin-fixed 0.5-1.0 ug/mL for 30 min at RT, Optimal dilution for a specific application should be determined by user, Western blotting 0.5-1.0 ug/mL

Positive control

HepG2 or A431 cells. Skin or Squamous cell carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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