It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
| Conjugation | Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP |
|---|---|
| Size | 50 uL, 100 uL, 250 uL, 500 uL |
| Supplied As | Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA |
| Antibody type | Primary |
| Host species | Mouse |
| Reactivity (target) | Cytokeratin 5/8 (C-50) |
| Clonality | Monoclonal |
| Isotype | IgM, kappa |
| Clone | C-50 |
| Molecular weight | 58 kDa (CK5); 52.5 kDa (CK8) |
| Synonyms | |
| Entrez gene ID | 3852 (CK5); 3856 (CK8) |
| SwissProt | P13647 (CK5); P05787 (CK8) |
| Unigene | 433845 (CK5); 533782 & 708445 (CK8) |
| Immunogen | Cytoskeletal preparation from HeLa cells |
| Species reactivity | Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep |
| Applications | Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western |
| Cellular localization | Cytoplasmic |
| Application notes | Does not react with chicken , or Xenopus, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/mL PBS, 15 min at RT, Immunofluorescence 0.5-1.0 ug/mL, Immunohistology formalin-fixed 0.5-1.0 ug/mL for 30 min at RT, Optimal dilution for a specific application should be determined by user, Western blotting 0.5-1.0 ug/mL |
| Positive control | HepG2 or A431 cells. Skin or Squamous cell carcinoma |
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