Monoclonal anti Cytokeratin 8/18 (B22.1 + B23.1)

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Catalog #: BNUB1221
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Product Description

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.

Angus B et. al. J Path, 153:377-384, 1987. 2. Angus B et. al. J Path, 155:71-75, 198

Additional Information


Flow cytometry, Immunofluorescence, Western


Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP


50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type


Host species


Reactivity (target)

Cytokeratin 8/18 (B22.1 + B23.1)




IgG1, kappa


B22.1 + B23.1

Molecular weight

52.5 kDa (CK8); 45 kDa (CK18)

Entrez gene ID

3856 (CK8); 3875 (CK18)


P05787 (CK8); P05783 (CK18)


Cytoskeletal preparation from HeLa epitheliod carcinoma cells (B22.1) and PMC-42 breast carcinoma cells of human origin (B23.1)


533782 & 708445 (CK8); 406013 (CK18)

Species reactivity


Cellular localization


Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Western blotting 0.5-1.0 ug/mL

Positive control

MCF-7, HeLa, PMC-42 or A431 cells. Skin, Colon, lung or breast carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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